Document Detail


Segmented frequency-domain fluorescence lifetime measurements: minimizing the effects of photobleaching within a multi-component system.
MedLine Citation:
PMID:  17713846     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study introduces a newly developed frequency segmentation and recombination method for frequency-domain fluorescence lifetime measurements to address the effects of changing fractional contributions over time and minimize the effects of photobleaching within multi-component systems. Frequency segmentation and recombination experiments were evaluated using a two component system consisting of fluorescein and rhodamine B. Comparison of experimental data collected in traditional and segmented fashion with simulated data, generated using different changing fractional contributions, demonstrated the validity of the technique. Frequency segmentation and recombination was also applied to a more complex system consisting of pyrene with Suwannee River fulvic acid reference and was shown to improve recovered lifetimes and fractional intensity contributions. It was observed that photobleaching in both systems led to errors in recovered lifetimes which can complicate the interpretation of lifetime results. Results showed clear evidence that the frequency segmentation and recombination method reduced errors resulting from a changing fractional contribution in a multi-component system, and allowed photobleaching issues to be addressed by commercially available instrumentation.
Authors:
Hadi M Marwani; Mark Lowry; Patrick Keating; Isiah M Warner; Robert L Cook
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-08-23
Journal Detail:
Title:  Journal of fluorescence     Volume:  17     ISSN:  1053-0509     ISO Abbreviation:  J Fluoresc     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-11-07     Completed Date:  2008-02-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9201341     Medline TA:  J Fluoresc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  687-99     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA.
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MeSH Terms
Descriptor/Qualifier:
Benzopyrans / chemistry
Fluorescein
Fluorescent Dyes
Photobleaching*
Pyrenes / chemistry
Rhodamines
Spectrometry, Fluorescence / methods*
Chemical
Reg. No./Substance:
0/Benzopyrans; 0/Fluorescent Dyes; 0/Pyrenes; 0/Rhodamines; 129-00-0/pyrene; 14899-08-2/rhodamine B; 2321-07-5/Fluorescein; 479-66-3/fulvic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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