Document Detail


Segmental demyelination induced by cerebrospinal fluid of progressive spastic paraparesis: correlation with altered proteolytic parameters.
MedLine Citation:
PMID:  8873117     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Progressive spastic paraparesis (PSP) is a demyelinating disease of the central nervous system. We studied the ability of the cerebrospinal fluid (CSF) of patients to induce alterations in rat peroneal nerves, and to modify the proteolytic activity of trypsin in vitro. Subperineurial injection of native or heated CSF of patients induced segmental demyelination and other cytological alterations 5-7 days later, in the infiltrated zone, while proximal and distal regions were normal. The CSF of normal subjects did not induce demyelination, but upon heating, it did so. Trypsin was strongly inhibited by the normal CSF but upon heating, its inhibitory activity was replaced by a strong potentiation. In contrast, native and heated CSF of patients potentiated trypsin. Our findings indicate that (1) the normal CSF contains a thermostable factor that potentiates trypsin whose function is overruled by thermolabile protease inhibitors; (2) the CSF of PSP patients has a reduced inhibitory activity and a conserved ability to potentiate trypsin; and (3) the CSF is endowed with a pathogenic power that correlates with an unchecked potentiating activity. We propose that the imbalance of a protease system may play a role in the pathogenesis of PSP lesions.
Authors:
J Schmidt; J K Nien; A Scherson; E O Campos; L Cartier; J Alvarez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience letters     Volume:  214     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1997-01-10     Completed Date:  1997-01-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  1-4     Citation Subset:  IM; X    
Affiliation:
Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cerebrospinal Fluid / physiology
Demyelinating Diseases / etiology*,  pathology
Hot Temperature
Humans
Injections
Paraparesis, Tropical Spastic / cerebrospinal fluid*
Peptide Hydrolases / metabolism
Peroneal Nerve / pathology
Rats
Reference Values
Trypsin / pharmacology
Chemical
Reg. No./Substance:
EC 3.4.-/Peptide Hydrolases; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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