| Segmental assembly of fibronectin matrix requires rap1b and integrin α5. | |
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MedLine Citation:
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PMID: 23192979 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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BACKGROUND: During segmentation of the zebrafish embryo, inside-out signaling activates Integrin α5, which is necessary for somite border morphogenesis. The direct activator of Integrin α5 during this process is unknown. One candidate is Rap1b, a small monomeric GTPase implicated in Integrin activation in the immune system. RESULTS: Knockdown of rap1b, or overexpression of a dominant negative rap1b, causes a mild axis elongation defect in zebrafish. However, disruption of rap1b function in integrin α5(-/-) mutants results in a strong reduction in Fibronectin (FN) matrix assembly in the paraxial mesoderm and a failure in somite border morphogenesis along the entire anterior-posterior axis. Somite patterning appears unaffected, as her1 oscillations are maintained in single and double morphants/mutants, but somite polarity is gradually lost in itgα5(-/-) ; rap1b MO embryos. CONCLUSIONS: In itgα5(-/-) mutants, rap1b is required for proper somite border morphogenesis in zebrafish. The loss of somite borders is not a result of aberrant segmental patterning. Rather, somite boundary formation initiates but is not completed, due to the failure to assemble FN matrix along the nascent boundary. We propose a model in which Rap1b activates Integrin/Fibronectin receptors as part of an "inside-out" signaling pathway that promotes Integrin binding to FN, FN matrix assembly and subsequent stabilization of morphological somite boundaries. © 2012 Wiley Periodicals, Inc., a Wiley company. |
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Authors:
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Simone Lackner; Jamie Schwendinger-Schreck; Dörthe Jülich; Scott A Holley |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-11-29 |
Journal Detail:
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Title: Developmental dynamics : an official publication of the American Association of Anatomists Volume: - ISSN: 1097-0177 ISO Abbreviation: Dev. Dyn. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9201927 Medline TA: Dev Dyn Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
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Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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