| Seeing GroEL at 6 A resolution by single particle electron cryomicroscopy. | |
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MedLine Citation:
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PMID: 15242589 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We present a reconstruction of native GroEL by electron cryomicroscopy (cryo-EM) and single particle analysis at 6 A resolution. alpha helices are clearly visible and beta sheet density is also visible at this resolution. While the overall conformation of this structure is quite consistent with the published X-ray data, a measurable shift in the positions of three alpha helices in the intermediate domain is observed, not consistent with any of the 7 monomeric structures in the Protein Data Bank model (1OEL). In addition, there is evidence for slight rearrangement or flexibility in parts of the apical domain. The 6 A resolution cryo-EM GroEL structure clearly demonstrates the veracity and expanding scope of cryo-EM and the single particle reconstruction technique for macromolecular machines. |
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Authors:
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Steven J Ludtke; Dong-Hua Chen; Jiu-Li Song; David T Chuang; Wah Chiu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Structure (London, England : 1993) Volume: 12 ISSN: 0969-2126 ISO Abbreviation: Structure Publication Date: 2004 Jul |
Date Detail:
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Created Date: 2004-07-09 Completed Date: 2005-02-09 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 101087697 Medline TA: Structure Country: United States |
Other Details:
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Languages: eng Pagination: 1129-36 Citation Subset: IM |
Affiliation:
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National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030 USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chaperonin 60
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chemistry* Cryoelectron Microscopy / methods* Crystallography, X-Ray Protein Structure, Quaternary Protein Structure, Secondary |
| Grant Support | |
ID/Acronym/Agency:
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DK 26758/DK/NIDDK NIH HHS; P01 GM 064692/GM/NIGMS NIH HHS; P41 RR 02250/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chaperonin 60 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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