Document Detail


Seeing citrulline: development of a phenylglyoxal-based probe to visualize protein citrullination.
MedLine Citation:
PMID:  23030787     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Protein arginine deiminases (PADs) catalyze the hydrolysis of peptidyl arginine to form peptidyl citrulline. Abnormally high PAD activity is observed in a host of human diseases, but the exact role of protein citrullination in these diseases and the identities of specific citrullinated disease biomarkers remain unknown, largely because of the lack of readily available chemical probes to detect protein citrullination. For this reason, we developed a citrulline-specific chemical probe, rhodamine-phenylglyoxal (Rh-PG), which we show can be used to investigate protein citrullination. This methodology is superior to existing techniques because it possesses higher throughput and excellent sensitivity. Additionally, we demonstrate that this probe can be used to determine the kinetic parameters for a number of protein substrates, monitor drug efficacy, and identify disease biomarkers in an animal model of ulcerative colitis that displays aberrantly increased PAD activity.
Authors:
Kevin L Bicker; Venkataraman Subramanian; Alexander A Chumanevich; Lorne J Hofseth; Paul R Thompson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-10-03
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  134     ISSN:  1520-5126     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-17     Completed Date:  2013-03-06     Revised Date:  2014-04-08    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  17015-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / analysis,  metabolism
Citrulline / chemistry*
Hydrolases / analysis*,  metabolism
Kinetics
Mice
Molecular Probes / chemistry*
Molecular Structure
Phenylglyoxal / chemistry*,  metabolism
Rhodamines / blood,  chemistry
Grant Support
ID/Acronym/Agency:
CA151304/CA/NCI NIH HHS; GM079357/GM/NIGMS NIH HHS; R01 CA151304/CA/NCI NIH HHS; R01 GM079357/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Molecular Probes; 0/Rhodamines; 29VT07BGDA/Citrulline; EC 3.-/Hydrolases; EC 3.5.3.6/arginine deiminase; N45G3015PA/Phenylglyoxal
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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