| Seed-specific overexpression of antioxidant genes in Arabidopsis enhances oxidative stress tolerance during germination and early seedling growth. | |
| | |
MedLine Citation:
|
PMID: 20691023 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Enzymatic and non-enzymatic antioxidants play important roles in the tolerance of abiotic stress. To increase the resistance of seeds to oxidative stress, At2S3 promoter from Arabidopsis was used to achieve overexpression of the antioxidants in a seed-specific manner. This promoter was shown to be capable of driving the target gene to have a high level of expression in seed-related organs, including siliques, mature seeds, and early seedlings, thus making its molecular farming applications in plants possible. Subsequently, genes encoding Mn-superoxide dismutase (MSD1), catalase (CAT1), and homogentisate phytyltransferase (HPT1, responsible for the first committed reaction in the tocopherol biosynthesis pathway) were overexpressed in Arabidopsis under the control of the At2S3 promoter. Double overexpressers co-expressing two enzymes and triple overexpressers were produced by cross pollination. Mn-SOD and total CAT activities, as well as gamma-tocopherol content, significantly increased in the corresponding overproduction lines. Moreover, single MSD1-transgene, double, and triple overexpressers displayed remarkably enhanced oxidative stress tolerance compared to wild type during seed germination and early seedling growth. Interestingly, an increase in the total CAT activity was also observed in the single MSD1-transgenic lines as a result of MSD1 overexpression. Together, the combined increase in Mn-SOD and CAT activities in seeds plays an essential role in the improvement of antioxidant capacity at early developmental stage in Arabidopsis. |
| | |
Authors:
|
Dong-Mei Xi; Wu-Shuang Liu; Guo-Dong Yang; Chang-Ai Wu; Cheng-Chao Zheng |
Related Documents
:
|
22507173 - The galu gene expression in streptococcus pneumonia. 17021803 - Differential expression of the ascorbate oxidase multigene family during fruit developm... 8804863 - A mutation in endothelin-b receptor gene causes myenteric aganglionosis and coat color ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Plant biotechnology journal Volume: 8 ISSN: 1467-7652 ISO Abbreviation: Plant Biotechnol. J. Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-08-09 Completed Date: 2010-10-28 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101201889 Medline TA: Plant Biotechnol J Country: England |
Other Details:
|
Languages: eng Pagination: 796-806 Citation Subset: IM |
Affiliation:
|
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alkyl and Aryl Transferases
/
genetics,
metabolism Antioxidants / metabolism* Arabidopsis / enzymology, genetics* Arabidopsis Proteins / genetics, metabolism Catalase / genetics, metabolism Gene Expression Regulation, Plant Germination* Oxidative Stress* Plants, Genetically Modified / enzymology, genetics Promoter Regions, Genetic RNA, Plant / genetics Seedling / growth & development* Superoxide Dismutase / genetics, metabolism Transgenes |
| Chemical | |
Reg. No./Substance:
|
0/Antioxidants; 0/Arabidopsis Proteins; 0/RNA, Plant; EC 1.11.1.6/Catalase; EC 1.15.1.1/Superoxide Dismutase; EC 2.5.-/Alkyl and Aryl Transferases; EC 2.5.-/HPT1 protein, Arabidopsis |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Overexpression of hydroperoxide lyase gene in Nicotiana benthamiana using a viral vector system.
Next Document: PET/CT today and tomorrow in veterinary cancer diagnosis and monitoring: fundamentals, early results...