| Sedimentation of lung-derived phospholipid during low-speed centrifugation of amniotic fluid. | |
| | |
MedLine Citation:
|
PMID: 7273402 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In most methods proposed for the assessment of fetal lung maturity, amniotic fluid is subjected to a preliminary low-speed centrifugation in an attempt to separate whole cells and cell debris from lung-derived surfactant phospholipid (lamellar body phospholipid). However, because lamellar body phospholipid is present in amniotic fluid in a membranous or particulate form, it is also partly sedimented by this procedure. The sedimentation of total phospholipid and lamellar body phospholipid by low-speed centrifugation has been quantitated for 49 samples of amniotic fluid from pregnancies of 30--41 weeks gestation. Isopycnic density-gradient centrifugation in a small air-driven ultracentrifuge was used to isolate lamellar body fractions from whole and centrifuged amniotic fluid. Centrifugation for 5 min at 1000 x g removed 0--70% of total phospholipid or lamellar body phospholipid, the mean values being 34 or 29%, respectively. There was an appreciable increase in lamellar body phospholipid relative to total phospholipid as a result of centrifugation in only 51% of the samples. In general, the effects of centrifugation were not related to gestational age of the fetus or the state of maturity of its lungs. |
| | |
Authors:
|
C G Duck-Chong; L M Brown; W J Hensley |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Clinical chemistry Volume: 27 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 1981 Aug |
Date Detail:
|
Created Date: 1981-11-18 Completed Date: 1981-11-18 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1424-6 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amniotic Fluid
/
analysis* Centrifugation / methods Female Gestational Age Humans Lung / embryology* Microscopy, Electron Phospholipids / analysis* Pregnancy Pregnancy Complications |
| Chemical | |
Reg. No./Substance:
|
0/Phospholipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Radioimmunoassay for plasma thromboxane B2.
Next Document: Spectrophotometry of tissue thromboplastin in cerebrospinal fluid.