| Secretory products of guinea pig epicardial fat induce insulin resistance and impair primary adult rat cardiomyocyte function. | |
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MedLine Citation:
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PMID: 21143387 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Epicardial adipose tissue has been implicated in the development of heart disease. Nonetheless, the crosstalk between factors secreted from epicardial adipose tissue and cardiomyocytes has not been studied. Here, we examined the effect of factors secreted from epicardial adipose tissue on contractile function and insulin signalling in primary rat cardiomocytes. Epicardial and subcutaneous adipose tissue were isolated from guinea pigs fed a high-fat (HFD) or standard diet. HFD-feeding for 6 months induced glucose intolerance, and decreased fractional shortening and ejection fraction (all P< 0.05). Conditioned media (CM) generated from epicardial and subcutaneous adipose tissue explants were subjected to cytokine profiling using antibody arrays, or incubated with cardiomyocytes to assess the effects on insulin action and contractile function. Eleven factors were differentially secreted by epicardial adipose tissue when compared to subcutaneous adipose tissue. Furthermore, secretion of 30 factors by epicardial adipose tissue was affected by HFD-feeding. Most prominently, activin A-immunoreactivity was 6.4-fold higher in CM from HFD- versus standard diet-fed animals and, 2-fold higher in epicardial versus subcutaneous adipose tissue. In cardiomyocytes, CM from epicardial adipose tissue of HFD-fed animals increased SMAD2-phosphorylation, a marker for activin A-signalling, decreased SERCA2a expression, and reduced insulin-mediated phosphorylation of Akt-Ser473 versus CM from subcutaneous adipose tissue and standard diet-fed animals. Finally, CM from epicardial adipose tissue of HFD-fed animals as compared to CM from the other groups markedly reduced sarcomere shortening and cytosolic Ca(2+) -fluxes in cardiomyocytes. These data provide evidence for an interaction between factors secreted from epicardial adipose tissue and cardiomyocyte function. |
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Authors:
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Sabrina Greulich; Daniella Herzfeld De Wiza; Sebastian Preilowksi; Zhaoping Ding; Heidi Mueller; Dominique Langin; Kornelia Jaquet; D Margriet Ouwens; Juergen Eckel |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2010-12-9 |
Journal Detail:
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Title: Journal of cellular and molecular medicine Volume: - ISSN: 1582-4934 ISO Abbreviation: - Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101083777 Medline TA: J Cell Mol Med Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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2010. |
Affiliation:
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Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Centre, Düsseldorf, Germany St. Josef-Hospital/Bergmannsheil, Clinic of the Ruhr-University, Bochum, Germany Institute of Cardiovascular Physiology, Heinrich Heine University, Düsseldorf, Germany Inserm, U858, University of Toulouse, UPS, Obesity Research Laboratory, Rangueil Institute of Molecular Medicine and CHU de Toulouse, Biochemistry Laboratory, Toulouse, France. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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