| Secretory production system of bionanocapsules using a stably transfected insect cell line. | |
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MedLine Citation:
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PMID: 16847600 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bionanocapsules (BNCs) are hollow nanoscale particles composed of L protein of the hepatitis B virus surface antigen that represent specific affinity for human hepatocytes. BNCs can transfer genes and drugs into human hepatocytes efficiently and specifically. BNC can be expressed in yeast cells. In this study, we developed a new L particle production system using a stably transfected insect cell line. For this purpose, we established a host-vector system using the Trichoplusia ni insect cell line. L particles were efficiently secreted by the overexpression of the L protein, which was fused to the secretion signal peptide. The concentration of L particles was reached approximately 1.7 microg/ml in 5 days during cultivation in a serum-free medium without antibiotic selective pressure. The production of L particles was maintained for at least 75 days. The secretory production of L particles facilitated their easy purification by chromatography. Furthermore, it was demonstrated that purified L particles can transfect only human hepatocytes. Therefore, an insect cell expression system is an attractive tool for the production of BNC. |
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Authors:
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Takuya Shishido; Masaru Muraoka; Masakazu Ueda; Masaharu Seno; Katsuyuki Tanizawa; Shun'ichi Kuroda; Hideki Fukuda; Akihiko Kondo |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-07-18 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 73 ISSN: 0175-7598 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2006-11-22 Completed Date: 2007-01-29 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 505-11 Citation Subset: IM |
Affiliation:
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Faculty of Engineering, Kobe University, Kobe 657-8501, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bees Cell Line Cell Line, Tumor Drosophila Electroporation Fluoresceins Genetic Vectors Hepatocytes Humans Melitten / chemistry Moths Nanocapsules* Promoter Regions, Genetic Protein Sorting Signals Recombinant Proteins / metabolism Sensitivity and Specificity Transfection* Viral Envelope Proteins / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Fluoresceins; 0/L protein, hepatitis B virus; 0/Nanocapsules; 0/Protein Sorting Signals; 0/Recombinant Proteins; 0/Viral Envelope Proteins; 1461-15-0/fluorexon; 20449-79-0/Melitten |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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