Document Detail


Secretory production system of bionanocapsules using a stably transfected insect cell line.
MedLine Citation:
PMID:  16847600     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bionanocapsules (BNCs) are hollow nanoscale particles composed of L protein of the hepatitis B virus surface antigen that represent specific affinity for human hepatocytes. BNCs can transfer genes and drugs into human hepatocytes efficiently and specifically. BNC can be expressed in yeast cells. In this study, we developed a new L particle production system using a stably transfected insect cell line. For this purpose, we established a host-vector system using the Trichoplusia ni insect cell line. L particles were efficiently secreted by the overexpression of the L protein, which was fused to the secretion signal peptide. The concentration of L particles was reached approximately 1.7 microg/ml in 5 days during cultivation in a serum-free medium without antibiotic selective pressure. The production of L particles was maintained for at least 75 days. The secretory production of L particles facilitated their easy purification by chromatography. Furthermore, it was demonstrated that purified L particles can transfect only human hepatocytes. Therefore, an insect cell expression system is an attractive tool for the production of BNC.
Authors:
Takuya Shishido; Masaru Muraoka; Masakazu Ueda; Masaharu Seno; Katsuyuki Tanizawa; Shun'ichi Kuroda; Hideki Fukuda; Akihiko Kondo
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-07-18
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  73     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-11-22     Completed Date:  2007-01-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  505-11     Citation Subset:  IM    
Affiliation:
Faculty of Engineering, Kobe University, Kobe 657-8501, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bees
Cell Line
Cell Line, Tumor
Drosophila
Electroporation
Fluoresceins
Genetic Vectors
Hepatocytes
Humans
Melitten / chemistry
Moths
Nanocapsules*
Promoter Regions, Genetic
Protein Sorting Signals
Recombinant Proteins / metabolism
Sensitivity and Specificity
Transfection*
Viral Envelope Proteins / genetics*,  metabolism
Chemical
Reg. No./Substance:
0/Fluoresceins; 0/L protein, hepatitis B virus; 0/Nanocapsules; 0/Protein Sorting Signals; 0/Recombinant Proteins; 0/Viral Envelope Proteins; 1461-15-0/fluorexon; 20449-79-0/Melitten

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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