| Secretory phospholipase A2 induces apoptosis through TNF-alpha and cytochrome c-mediated caspase cascade in murine macrophage RAW 264.7 cells. | |
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MedLine Citation:
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PMID: 16564042 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phospholipase A2 (PLA2) is an esterase that cleaves the sn-2 ester bond in glycerophospholipids, thereby releasing free fatty acids and lysophospholipids. In addition to the apoptotic activity of cytosolic PLA2 and Ca2+-independent PLA2, recent studies showed that secretory PLA2 (sPLA2) also play a role in apoptosis. However, the details of molecular mechanism have not been fully elucidated. Our data demonstrated that group IB PLA (IB PLA2)-exposed murine macrophage 264.7 cells showed characteristic features of apoptosis such as morphological changes, DNA laddering, staining positive for propidium iodide (PI) as well as Annexin V and activation of caspases and subsequent cleavage of poly (ADP-ribose) polymerase (PARP) in dose- and time-dependent manner. Moreover, IB PLA2 was found to elicit tumor necrosis factor (TNF)-alpha production and release of cytochrome c, suggesting that IB PLA2 exerts its apoptotic activity via the induction of TNF-alpha production and cytochrome c release, which results in triggering the activation of caspase cascade and PARP cleavage. |
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Authors:
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ChuHee Lee; Dae-Weon Park; Jingu Lee; Tae-Il Lee; Young-Jo Kim; Yun-Sik Lee; Suk-Hwan Baek |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-02-28 |
Journal Detail:
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Title: European journal of pharmacology Volume: 536 ISSN: 0014-2999 ISO Abbreviation: Eur. J. Pharmacol. Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-04-17 Completed Date: 2006-07-25 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1254354 Medline TA: Eur J Pharmacol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 47-53 Citation Subset: IM |
Affiliation:
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Aging-Associated Vascular Disease Research Center, Department of Biochemistry and Molecular Biology, College of Medicine, Yeungnam University, Daegu 705-717, South Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies / pharmacology Apoptosis / drug effects* Blotting, Western Caspase 3 Caspase 7 Caspases / antagonists & inhibitors, metabolism* Cell Line Cysteine Proteinase Inhibitors / pharmacology Cytochromes c / secretion* Dose-Response Relationship, Drug Enzyme Activation / drug effects Group IB Phospholipases A2 Macrophages / cytology, drug effects*, metabolism Mice Phospholipases A / pharmacology* Phospholipases A2 Poly(ADP-ribose) Polymerases / metabolism Swine Time Factors Tumor Necrosis Factor-alpha / biosynthesis*, immunology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Cysteine Proteinase Inhibitors; 0/Tumor Necrosis Factor-alpha; 9007-43-6/Cytochromes c; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Group IB Phospholipases A2; EC 3.1.1.4/Phospholipases A2; EC 3.1.1.4/Pla2g1b protein, mouse; EC 3.4.22.-/Casp3 protein, mouse; EC 3.4.22.-/Casp7 protein, mouse; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 7; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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