Document Detail


Secretory differentiation of serially passaged normal human nasal epithelial cells by retinoic acid: expression of mucin and lysozyme.
MedLine Citation:
PMID:  10855573     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to subculture normal human nasal epithelial (NHNE) cells without compromising their ability to differentiate into secretory and ciliated cells and to study the effect of retinoic acid on mucous and serous secretions in passaged cells and to compare the expression of mucin and lysozyme in cultured cells with those in in vivo nasal epithelium. The subcultured cells were tested after every passage for secretory differentiation in air-liquid interface cultures. The cultured NHNE cells secreted mucin and lysozyme. The cells became squamous and mucin secretion decreased when retinoic acid was deleted from the culture media. Cells from passage 1 through passage 2 remained able to differentiate into mucous or squamous cells. Mucin gene 4 (MUC4), MUC5AC, MUC7, MUC8, and lysozyme messenger RNAs were expressed in passage 2 NHNE cells. In conclusion, passage 2 NHNE cell cultures retain features of normal epithelium and are suitable for many studies of upper airway cell biology.
Authors:
J H Yoon; K S Kim; S S Kim; J G Lee; I Y Park
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Annals of otology, rhinology, and laryngology     Volume:  109     ISSN:  0003-4894     ISO Abbreviation:  Ann. Otol. Rhinol. Laryngol.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-06-26     Completed Date:  2000-06-26     Revised Date:  2006-05-15    
Medline Journal Info:
Nlm Unique ID:  0407300     Medline TA:  Ann Otol Rhinol Laryngol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  594-601     Citation Subset:  AIM; IM    
Affiliation:
Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Cell Differentiation / drug effects*
Cells, Cultured
Epithelial Cells / drug effects,  secretion
Gene Expression Regulation, Enzymologic / drug effects
Humans
Mucins / genetics*
Muramidase / genetics*
Nasal Mucosa / drug effects*,  secretion
RNA, Messenger / genetics
Reverse Transcriptase Polymerase Chain Reaction
Secretory Rate / drug effects*
Tretinoin / pharmacology*
Chemical
Reg. No./Substance:
0/Mucins; 0/RNA, Messenger; 302-79-4/Tretinoin; EC 3.2.1.17/Muramidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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