| Secretory leukocyte protease inhibitor inhibits neutrophil apoptosis. | |
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MedLine Citation:
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PMID: 21077989 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background and objective: The secretory leukocyte protease inhibitor (SLPI) is a major anti-elastase barrier at the epithelial surfaces of upper respiratory tract. In addition to its anti-protease activity, SLPI has been shown to express anti-bacterial, anti-viral and anti-inflammatory properties. Methods: We measured SLPI concentration in nasal lavage fluid of healthy volunteers after challenge with endotoxin (LPS) and evaluated SLPI effects in vitro on neutrophil chemotaxis, adhesion, cytokine (IL-8) release and apoptosis. Results: SLPI concentration in nasal lavage (n = 9) 2, 6 and 24 h after the challenge with LPS (25 µg) increased from 32% to 238% compared with baseline (226 ± 71 ng/mL). In vitro, SLPI (20-80 µg/mL) induced neutrophil chemotaxis (sixfold, P < 0.001) and decreased neutrophil apoptosis by 73% (P = 0.006), relative to controls. However, SLPI had no affect on IL-8 release or neutrophil adhesion to fibronectin. SLPI-positive immunoreactivity was co-localized with neutrophils in lung specimens from patients with COPD. Conclusions: Our findings indicate upregulation of SLPI in response to LPS in nasal secretions and show anti-apoptotic effects of SLPI in primary human neutrophils suggesting a new role of SLPI during neutrophilic inflammation. |
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Authors:
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Devipriya Subramaniyam; Camilla Hollander; Ulla Westin; Jonas Erjefält; Tim Stevens; Sabina Janciauskiene |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Respirology (Carlton, Vic.) Volume: 16 ISSN: 1440-1843 ISO Abbreviation: Respirology Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9616368 Medline TA: Respirology Country: Australia |
Other Details:
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Languages: eng Pagination: 300-7 Citation Subset: IM |
Copyright Information:
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© 2011 The Authors. Respirology © 2011 Asian Pacific Society of Respirology. |
Affiliation:
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Department of Respiratory Medicine, Hannover medical School, Hannover, GermanyDepartments of Otolaryngology and Head and Neck Surgery Clinical Sciences, Lund University, University Hospital Malmö, Malmö Astrazeneca R&D, Lund, Sweden. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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