Document Detail


Secretory IgA- and IgG-coated bacteria in the nasopharynx of children. An immunofluorescence study.
MedLine Citation:
PMID:  1763637     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bacterial samples were obtained from the posterior wall of the nasopharynx (NPH) of 18 healthy children (age range 1 to 14 years, 10 males, 8 females) and subjected for immunofluorescence studies using fluorescein-labelled goat anti-human IgG and goat anti-human SIgA antibodies. Quantitative and qualitative bacteriological analyses were performed simultaneously. IgG-coated bacteria in the NPH were observed in 1-year-old subjects and opsonized bacteria seemed to increase in numbers and fluorescence intensity with increasing age. SIgA-coated bacteria appeared later, and intensely fluorescing bacteria were not seen until the age of 3 to 6 years. Thirteen out of the 18 children harboured middle ear pathogens in the NPH, but generally speaking non-pathogens dominated the bacterial flora. Immunoglobulins are of the utmost importance for the mucosal defence system, including protection against bacterial attachment to the epithelial cells (SIgA), and bacteriolysis and opsonization of the bacteria (IgG).
Authors:
L E Stenfors; S Räisänen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta oto-laryngologica     Volume:  111     ISSN:  0001-6489     ISO Abbreviation:  Acta Otolaryngol.     Publication Date:  1991  
Date Detail:
Created Date:  1992-02-13     Completed Date:  1992-02-13     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0370354     Medline TA:  Acta Otolaryngol     Country:  SWEDEN    
Other Details:
Languages:  eng     Pagination:  1139-45     Citation Subset:  IM    
Affiliation:
Department of Otolaryngology, University of Tromsö, Norway.
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MeSH Terms
Descriptor/Qualifier:
Child
Child, Preschool
Female
Fluorescent Antibody Technique
Humans
Immunoglobulin A, Secretory / immunology*
Immunoglobulin G / immunology*
Male
Mucous Membrane / immunology
Nasopharynx / microbiology*
Opsonin Proteins / immunology
Otitis Media / microbiology
Phagocytosis
Chemical
Reg. No./Substance:
0/Immunoglobulin A, Secretory; 0/Immunoglobulin G; 0/Opsonin Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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