Document Detail


Secretomic analysis identifies alpha-1 antitrypsin (A1AT) as a required protein in cancer cell migration, invasion, and pericellular fibronectin assembly for facilitating lung colonization of lung adenocarcinoma cells.
MedLine Citation:
PMID:  22896658     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Metastasis is a major obstacle that must be overcome for the successful treatment of lung cancer. Proteins secreted by cancer cells may facilitate the progression of metastasis, particularly within the phases of migration and invasion. To discover metastasis-promoting secretory proteins within cancer cells, we used the label-free quantitative proteomics approach and compared the secretomes from the lung adenocarcinoma cell lines CL1-0 and CL1-5, which exhibit low and high metastatic properties, respectively. By employing quantitative analyses, we identified 660 proteins, 68 of which were considered to be expressed at different levels between the two cell lines. High levels of A1AT were secreted by CL1-5, and the roles of A1AT in the influence of lung adenocarcinoma metastasis were investigated. Molecular and pathological confirmation demonstrated that altered expression of A1AT correlates with the metastatic potential of lung adenocarcinoma. The migration and invasion properties of CL1-5 cells were significantly diminished by reducing the expression and secretion of their A1AT proteins. Conversely, the migration and invasion properties of CL1-0 cells were significantly increased through the overexpression and secretion of A1AT proteins. Furthermore, the assembly levels of the metastasis-promoting pericellular fibronectin (FN1), which facilitates colonization of lung capillary endothelia by adhering to the cell surface receptor dipeptidyl peptidase IV (DPP IV), were higher on the surfaces of suspended CL1-5 cells than on those of the CL1-0 cells. This discovery reflects previous findings in breast cancer. In line with this finding, FN1 assembly and the lung colonization of suspended CL1-5 cells were inhibited when endogenous A1AT protein was knocked down using siRNA. The major thrust of this study is to demonstrate the effects of coupling the label-free proteomics strategy with the secretomes of cancer cells that differentially exhibit invasive and metastatic properties. This provides a new opportunity for the effective identification of metastasis-associated proteins that are secreted by cancer cells and promote experimental metastasis.
Authors:
Ying-Hua Chang; Shu-Hui Lee; I-Chuang Liao; Shin-Huei Huang; Hung-Chi Cheng; Pao-Chi Liao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-15
Journal Detail:
Title:  Molecular & cellular proteomics : MCP     Volume:  11     ISSN:  1535-9484     ISO Abbreviation:  Mol. Cell Proteomics     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-09     Completed Date:  2013-04-23     Revised Date:  2013-11-05    
Medline Journal Info:
Nlm Unique ID:  101125647     Medline TA:  Mol Cell Proteomics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1320-39     Citation Subset:  IM    
Affiliation:
Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University and Hospital, Tainan, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / pathology*,  secretion*
Cell Line, Tumor
Cell Movement* / drug effects
Cell Proliferation / drug effects
Culture Media, Conditioned / pharmacology
Fibronectins / metabolism*
Humans
Immunohistochemistry
Lung / metabolism,  pathology*
Lung Neoplasms / pathology*,  secretion*
Male
Middle Aged
Neoplasm Invasiveness
Neoplasm Metastasis
Neoplasm Proteins / secretion*
Polymerization / drug effects
Reproducibility of Results
alpha 1-Antitrypsin / metabolism*
Chemical
Reg. No./Substance:
0/Culture Media, Conditioned; 0/Fibronectins; 0/Neoplasm Proteins; 0/alpha 1-Antitrypsin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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