Document Detail

Secretion of saliva in X-irradiated rat submandibular glands.
MedLine Citation:
PMID:  12600238     Owner:  NLM     Status:  MEDLINE    
The mechanism of radiation-induced dysfunction in rat submandibular glands was investigated at the cellular level. After X irradiation (single dose, 15 Gy), a vacuolation in the acinar cells or an enlargement of the acinar lumen was observed as a typical morphological change for 2 weeks. As observed using a video-enhanced contrast differential interference contrast (VEC-DIC) microscope, exocytosis and shrinkage of the acinar cells induced by application of pilocarpine (100 microM) were markedly suppressed for 5 days and then recovered to 80% of the control levels. Using an immunohistochemical method, no significant change was observed in amylase distribution, but a marked loss of aquaporin 5 was found in the acinar cells after the irradiation. The extent and time course of pilocarpine-induced mobilization of intracellular Ca(2+) did not change after the irradiation. We conclude that radiation-induced dysfunction in the salivary glands is due to an impairment of exocytosis and a reduction of water secretion. The loss of aquaporin 5 and possibly other membrane-fusion proteins in acinar cells may be the major mechanism underlying such a dysfunction.
Koji Takagi; Kazue Yamaguchi; Takashi Sakurai; Tetsuya Asari; Kenji Hashimoto; Susumu Terakawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Radiation research     Volume:  159     ISSN:  0033-7587     ISO Abbreviation:  Radiat. Res.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-02-25     Completed Date:  2003-04-25     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  351-60     Citation Subset:  IM; S    
Copyright Information:
Copyright 2003 by Radiation Research Society
Department of Dentistry and Oral and Maxillofacial Surgery, Hamamatsu University School of Medicine, Hamamatsu, 431-3192 Japan.
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MeSH Terms
Amylases / metabolism
Aquaporin 5
Aquaporins / metabolism
Calcium / metabolism
Cell Movement
Membrane Proteins*
Microscopy, Confocal
Microscopy, Electron
Microscopy, Video
Pilocarpine / pharmacology
Rats, Sprague-Dawley
Saliva / radiation effects*,  secretion*
Salivary Glands / metabolism
Submandibular Gland / radiation effects*,  secretion*
Time Factors
Reg. No./Substance:
0/Aqp5 protein, rat; 0/Aquaporin 5; 0/Aquaporins; 0/Membrane Proteins; 7440-70-2/Calcium; 92-13-7/Pilocarpine; EC 3.2.1.-/Amylases

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