| Secretion is required for late events in the cell-fusion pathway of mating yeast. | |
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MedLine Citation:
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PMID: 20460435 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Secretory vesicles accumulate adjacent to the contact site between the two cells of a yeast mating pair before they fuse, but there is no direct evidence that secretion is required to complete fusion. In this study, temperature-sensitive secretion (sec(ts)) mutants were used to investigate the role of secretion in yeast cell fusion. Cell fusion arrested less than 5 minutes after inhibiting secretion. This rapid fusion arrest was not an indirect consequence of reduced mating pheromone signaling, mating-pair assembly or actin polarity. Furthermore, secretion was required to complete cell fusion when it was transiently inhibited by addition and removal of the lipophilic styryl dye, FM4-64. These results indicate that ongoing secretion is required for late events in the cell-fusion pathway, which include plasma-membrane fusion and the completion of cell-wall remodeling, and they demonstrate a just-in-time delivery mechanism for the cell-fusion machinery. |
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Authors:
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Eric Grote |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-11 |
Journal Detail:
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Title: Journal of cell science Volume: 123 ISSN: 1477-9137 ISO Abbreviation: J. Cell. Sci. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-20 Completed Date: 2010-09-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0052457 Medline TA: J Cell Sci Country: England |
Other Details:
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Languages: eng Pagination: 1902-12 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe Street, Baltimore, MD 21205. egrote@jhsph.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Cultured Genetic Complementation Test Membrane Fusion / drug effects, genetics Membrane Proteins / genetics Mutation / genetics Pyridinium Compounds / pharmacology Quaternary Ammonium Compounds / pharmacology Reproduction Saccharomyces cerevisiae Proteins / genetics, metabolism* Secretory Pathway Secretory Vesicles / metabolism* Temperature Vesicular Transport Proteins / genetics, metabolism* Yeasts / physiology* beta-Galactosidase / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/FM 4-64; 0/FUS1 protein, S cerevisiae; 0/Membrane Proteins; 0/PRM1 protein, S cerevisiae; 0/Pyridinium Compounds; 0/Quaternary Ammonium Compounds; 0/SEC6 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Vesicular Transport Proteins; EC 3.2.1.23/beta-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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