| Secretion of phospholipase A1 by bone marrow-derived macrophages. | |
| | |
MedLine Citation:
|
PMID: 4041474 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Bone marrow-derived macrophages contain phospholipase activity of the A1 and A2 types, active at acid or neutral pH and with different specificities for the fatty acid to be liberated. In contrast to this variety, only one single phospholipase could be detected in extracellular fluids of these cells. Surprisingly, this phospholipase was of the A1 type and active at about pH 8. It exhibited a restricted substrate specificity in that, of the various substrates tested, only phosphatidylcholine containing palmitic acid in position 2 was degraded. This total restriction was not detected with phosphatidylethanolamine substrates. In addition to phospholipase A1, extracellular fluids exhibited lipase activity. A modulation of enzyme secretion could not be achieved by lymphokines or phorbol esters. However, release could be blocked by treating cells with cycloheximide (5 micrograms/ml) or tunicamycin (0.5 micrograms/ml). Phospholipase A1 was also released by thioglycollate-induced peritoneal macrophages. |
| | |
Authors:
|
B Schmidt; K Hansen; E Ferber |
Related Documents
:
|
12686484 - Structural analysis of phosphatidylcholines by post-source decay matrix-assisted laser ... 2988614 - Phase transitions and fatty acid spin label behavior in interdigitated lipid phases ind... 17466344 - Long- and short-term phosphate deprivation in bean roots: plasma membrane lipid alterat... 2493094 - Uptake and hormonally induced hydrolysis of [3h]phosphatidyl choline in the isolated ra... 7874864 - Modulation of cytokine production in vivo by dietary essential fatty acids in patients ... 12602604 - Electrically enhanced photodegradation of an azodye (acid orange ii) using a pt/tio2 fi... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: 836 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1985 Oct |
Date Detail:
|
Created Date: 1985-10-30 Completed Date: 1985-10-30 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 312-20 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Bone Marrow / enzymology* Carbon Radioisotopes Cells, Cultured Female Kinetics Macrophages / enzymology* Mice Mice, Inbred C57BL Phospholipases / secretion* Phospholipases A / metabolism, secretion* Phospholipases A1 Substrate Specificity |
| Chemical | |
Reg. No./Substance:
|
0/Carbon Radioisotopes; EC 3.1.-/Phospholipases; EC 3.1.1.-/Phospholipases A; EC 3.1.1.32/Phospholipases A1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The time-course of lipid biosynthesis in horse skin.
Next Document: The availability of different sources of cholesterol for bile acid synthesis by cultured chick embry...