Document Detail

Secretion of phospholipase A1 by bone marrow-derived macrophages.
MedLine Citation:
PMID:  4041474     Owner:  NLM     Status:  MEDLINE    
Bone marrow-derived macrophages contain phospholipase activity of the A1 and A2 types, active at acid or neutral pH and with different specificities for the fatty acid to be liberated. In contrast to this variety, only one single phospholipase could be detected in extracellular fluids of these cells. Surprisingly, this phospholipase was of the A1 type and active at about pH 8. It exhibited a restricted substrate specificity in that, of the various substrates tested, only phosphatidylcholine containing palmitic acid in position 2 was degraded. This total restriction was not detected with phosphatidylethanolamine substrates. In addition to phospholipase A1, extracellular fluids exhibited lipase activity. A modulation of enzyme secretion could not be achieved by lymphokines or phorbol esters. However, release could be blocked by treating cells with cycloheximide (5 micrograms/ml) or tunicamycin (0.5 micrograms/ml). Phospholipase A1 was also released by thioglycollate-induced peritoneal macrophages.
B Schmidt; K Hansen; E Ferber
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  836     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1985 Oct 
Date Detail:
Created Date:  1985-10-30     Completed Date:  1985-10-30     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  312-20     Citation Subset:  IM    
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MeSH Terms
Bone Marrow / enzymology*
Carbon Radioisotopes
Cells, Cultured
Macrophages / enzymology*
Mice, Inbred C57BL
Phospholipases / secretion*
Phospholipases A / metabolism,  secretion*
Phospholipases A1
Substrate Specificity
Reg. No./Substance:
0/Carbon Radioisotopes; EC 3.1.-/Phospholipases; EC 3.1.1.-/Phospholipases A; EC A1

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