Document Detail


Secretion of a lysophospholipase D activity by adipocytes: involvement in lysophosphatidic acid synthesis.
MedLine Citation:
PMID:  12032165     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of the present work was to depict the metabolic pathways involved in extracellular production of lysophosphatidic acid (LPA) by adipocytes. LPA was followed by quantifying the accumulation of LPA in the incubation medium (conditioned medium, CM) of 3T3F442A adipocytes or human adipose tissue explants using a radioenzymatic assay. Surprisingly, after separation from the cells, the amount of LPA present in CM could be significantly increased by further incubation at 37 degrees C. This suggested the presence of a LPA-synthesizing activity (LPA-SA) in CM. LPA-SA appeared as a soluble activity which was inhibited by divalent ion chelators EDTA and phenanthrolin. The effect of EDTA was preferentially reverted by CoCl2, as described for a lysophospholipase D (lyso-PLD) activity previously identified in rat plasma. LPA concentration could also be increased by treatment with a bacterial PLD, demonstrating the presence of PLD-sensitive LPA precursors (mainly lysophosphatidylcholine) in adipocyte CM. LPA-SA could be increased by the addition of exogenous lysophosphatidylcholine, lysophosphatidylglycerol, or lyso-platelet activating factor, demonstrating that LPA-SA resulted from the action of a lyso-PLD. LPA-SA was not inhibited, but rather activated, by primary alcohol (ethanol and 1-butanol), suggesting that adipocyte lyso-PLD was not a classical PLD. Finally, LPA-SA was found to be weaker in CM of undifferentiated adipocyte (preadipocytes) compared with CM of differentiated adipocytes. In conclusion, our results reveal the existence of a secreted lyso-PLD activity regulated during adipocyte-differentiation and involved in extra cellular production of synthesis of LPA by adipocytes.
Authors:
Stéphane Gesta; Marie-Françoise Simon; Astrid Rey; David Sibrac; Alexia Girard; Max Lafontan; Philippe Valet; Jean Sébastien Saulnier-Blache
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of lipid research     Volume:  43     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-05-28     Completed Date:  2003-01-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  904-10     Citation Subset:  IM    
Affiliation:
INSERM U317, Institut Louis Bugnard, Université Paul Sabatier, CHU Rangueil, Batiment L3, Toulouse cedex 04, France.
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / secretion*
Animals
Cells, Cultured
Culture Media, Conditioned / metabolism
Edetic Acid / metabolism
Female
Hot Temperature
Humans
Lysophosphatidylcholines / metabolism
Lysophospholipids / biosynthesis*
Phenanthrolines / metabolism
Phosphoric Diester Hydrolases / secretion*
Chemical
Reg. No./Substance:
0/Culture Media, Conditioned; 0/Lysophosphatidylcholines; 0/Lysophospholipids; 0/Phenanthrolines; 60-00-4/Edetic Acid; EC 3.1.4.-/Phosphoric Diester Hydrolases; EC 3.1.4.39/alkylglycerophosphoethanolamine phosphodiesterase
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