| Secretion of immunoreactive beta-endorphin and GnRH by the pig uterus: effects of reproductive status and breed. | |
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MedLine Citation:
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PMID: 8283447 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Immunoreactive beta-endorphin (ir-BEND) and GnRH (ir-GnRH) have been identified in the pig uterus. The study reported here examined (i) possible biochemical differences between ir-BEND and ir-GnRH present in pig uterine fluids and standard synthetic peptides, and (ii) the secretory profiles of uterine ir-BEND and ir-GnRH during the oestrous cycle and early pregnancy for two breeds of pig, Large White and the highly prolific Chinese Meishan. Reverse phase (RP)-HPLC analysis of concentrated uterine fluids indicated that the majority of ir-BEND eluted with a hydrophobicity similar to that of synthetic BEND 1-31 (BEND) and alpha-N-acetylated BEND 1-31 (Nac-BEND), with a ratio of BEND to Nac-BEND of approximately 1.8:1. The RP-HPLC profiles of ir-GnRH demonstrated a major peak coeluting with synthetic GnRH, along with a minor peak eluting at the void volume. Total content of ir-BEND (pg per uterus) was affected by the interaction of breed with day (P < 0.001), but was independent of reproductive status. In Large White gilts, uterine fluid content of ir-BEND was higher (P < 0.05) on days 10 and 11 than on days 8, 12 and 14; however, in Meishan gilts, ir-BEND decreased from day 8 to days 10 and 11, and remained low on days 12 and 14. Compared to Meishan gilts, Large White gilts had higher ir-BEND concentrations on day 10 (P < 0.01), day 11 (P < 0.0001), day 12 (P < 0.01) and day 14 (P < 0.02).(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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W I Li; S Jiao; C L Chen; F W Bazer |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of reproduction and fertility Volume: 99 ISSN: 0022-4251 ISO Abbreviation: J. Reprod. Fertil. Publication Date: 1993 Sep |
Date Detail:
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Created Date: 1994-02-15 Completed Date: 1994-02-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376367 Medline TA: J Reprod Fertil Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 253-8 Citation Subset: IM |
Affiliation:
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Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens 30602. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Body Fluids / chemistry Chromatography, High Pressure Liquid Estrus / blood Female Gonadotropin-Releasing Hormone / chemistry, secretion* Pregnancy Reproduction / physiology* Swine / genetics, physiology* Uterus / secretion* beta-Endorphin / chemistry, secretion* |
| Chemical | |
Reg. No./Substance:
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33515-09-2/Gonadotropin-Releasing Hormone; 60617-12-1/beta-Endorphin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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