Document Detail


Secretion of green fluorescent protein from recombinant baculovirus-infected insect cells.
MedLine Citation:
PMID:  8831686     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Trichoplusia ni (High Five) and Spodoptera frugiperda (Sf21) cells were engineered for expression of epitope (Flag)-tagged signal peptide-green fluorescent protein (GFP) fusions to examine the suitability of GFP as a secretory marker. The recombinant baculovirus-infected cells became fluorescent, and the High Five cells but not Sf21 cells secreted GFP in the culture medium as detected by the presence in the culture supernatant of a Flag-immunoreactive 30-kDa species and the characteristic 510-nm GFP fluorescence peak. Signal peptides derived from ecdysteroid UDP-glucosyltransferase of Autographa californica nuclear polyhedrosis virus and from rat brain glutamate receptor were both able to promote secretion of GFP. GFP may thus be used as a research tool in the study of the secretory process in insect cells both in cell biology and in biotechnological applications.
Authors:
M L Laukkanen; C Oker-Blom; K Keinänen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  226     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1996-11-07     Completed Date:  1996-11-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  755-61     Citation Subset:  IM    
Affiliation:
VTT Biotechnology and Food Research, Espoo, Finland.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Baculoviridae
Base Sequence
Cell Line
DNA Primers
Gene Expression
Glucosyltransferases / biosynthesis
Green Fluorescent Proteins
Insects
Luminescent Proteins / biosynthesis*
Molecular Sequence Data
Polymerase Chain Reaction
Protein Sorting Signals / biosynthesis
Rats
Receptors, Glutamate / biosynthesis
Recombinant Fusion Proteins / biosynthesis*
Sequence Tagged Sites
Spodoptera
Transfection
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Luminescent Proteins; 0/Protein Sorting Signals; 0/Receptors, Glutamate; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins; EC 2.4.1.-/Glucosyltransferases; EC 2.4.1.-/ecdysteroid UDP-glucosyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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