Document Detail


Secretion of ghrelin from rat stomach ghrelin cells in response to local microinfusion of candidate messenger compounds: a microdialysis study.
MedLine Citation:
PMID:  17573135     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ghrelin is produced by A-like cells (ghrelin cells) in the mucosa of the acid-producing part of the stomach. The mobilization of ghrelin is stimulated by nutritional deficiency and suppressed by nutritional abundance. In an attempt to identify neurotransmitters and regulatory peptides that may contribute to the physiological, nutrient-related regulation of ghrelin secretion, we challenged the ghrelin cells in situ with a wide variety of candidate messengers, including known neurotransmitters (e.g. acetylcholine, catecholamines), candidate neurotransmitters (e.g. neuropeptides), local tissue hormones (e.g. serotonin, histamine, bradykinin, endothelin), circulating gut hormones (e.g. gastrin, CCK, GIP, neurotensin, PYY, secretin) and other circulating hormones/regulatory peptides (e.g. calcitonin, glucagon, insulin, PTH). Microdialysis probes were placed in the submucosa of the acid-producing part of the rat stomach. Three days later, the putative messenger compounds were administered via the microdialysis probe (reverse microdialysis) at a screening dose of 0.1 mmol l(-1) for regulatory peptides and 0.1 and 1 mmol l(-1) for amines and amino acids. The rats were awake during the experiments. The resulting microdialysate ghrelin concentration was monitored continuously for 3 h (radioimmunoassay), thereby revealing stimulators or inhibitors of ghrelin secretion. Dose-response curves were constructed for each candidate messenger that significantly (p<0.05) affected ghrelin mobilization at the screening dose. Peptides that showed a (non-significant) tendency to affect ghrelin release at the screening dose were also given at a dose of 0.3 or 1 mmol l(-1). Adrenaline, noradrenaline, endothelin and secretin stimulated ghrelin release, while somatostatin and GRP inhibited. Whether these agents act directly or indirectly on the ghrelin cells remains to be investigated. All other candidate messengers were without measurable effects, including acetylcholine, serotonin, histamine, GABA, aspartic acid, glutamic acid, glycine, VIP, PACAP, CGRP, substance P, NPY, PYY, PP, gastrin, CCK, GIP, insulin, glucagon, GLP and glucose.
Authors:
Charlotta Dornonville de la Cour; Per Norlén; Rolf Håkanson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-10
Journal Detail:
Title:  Regulatory peptides     Volume:  143     ISSN:  0167-0115     ISO Abbreviation:  Regul. Pept.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-08-13     Completed Date:  2007-11-06     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8100479     Medline TA:  Regul Pept     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  118-26     Citation Subset:  IM    
Affiliation:
Unit of Cellular and Molecular Pharmacology, Dept of Exp. Med, University of Lund, BMC F13, S-221 84 Lund, Sweden. CDDC@novonordisk.com
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MeSH Terms
Descriptor/Qualifier:
Amines / pharmacology
Amino Acids / pharmacology
Animals
Female
Gastric Inhibitory Polypeptide / pharmacology
Gastrins / pharmacology
Gastrointestinal Hormones / pharmacology
Ghrelin
Glucagon / pharmacology
Glucose / pharmacology
Histamine / pharmacology
Insulin / pharmacology
Microdialysis / methods*
Neuropeptides / pharmacology
Pancreatic Hormones / pharmacology
Peptide Hormones / metabolism*
Peptide YY / pharmacology
Rats
Rats, Sprague-Dawley
Stomach / cytology,  drug effects,  metabolism*
Chemical
Reg. No./Substance:
0/Amines; 0/Amino Acids; 0/Gastrins; 0/Gastrointestinal Hormones; 0/Ghrelin; 0/Neuropeptides; 0/Pancreatic Hormones; 0/Peptide Hormones; 106388-42-5/Peptide YY; 11061-68-0/Insulin; 50-99-7/Glucose; 51-45-6/Histamine; 59392-49-3/Gastric Inhibitory Polypeptide; 9007-92-5/Glucagon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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