Document Detail

Secretion of fructosyltransferase by Streptococcus salivarius involves the sucrose-dependent release of the cell-bound form.
MedLine Citation:
PMID:  2141067     Owner:  NLM     Status:  MEDLINE    
Three strains of Streptococcus salivarius including a recent clinical isolate were found to possess Ca2(+)-dependent fructosyltransferase (FTF) activity. The extracellular FTF activity of cells grown on sucrose increased as much as 9-fold compared with cells grown on either glucose, fructose or galactose. This increase in activity was due not to induction of FTF by sucrose, but to the release of the cell-bound form of the enzyme. Studies with washed cells of S. salivarius ATCC 25975 showed that the extent of release of the cell-bound FTF activity was dependent upon the sucrose concentration up to 4 mM, at which concentration maximum release (95%) of cell-bound FTF occurred. Several lines of evidence suggested that either substrate binding or de novo synthesis of fructan is required for the release of the cell-bound FTF activity.
C P Milward; N A Jacques
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of general microbiology     Volume:  136     ISSN:  0022-1287     ISO Abbreviation:  J. Gen. Microbiol.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-07-13     Completed Date:  1990-07-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375371     Medline TA:  J Gen Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  165-9     Citation Subset:  IM    
Institute of Dental Research, United Dental Hospital of Sydney, Surry Hills, New South Wales, Australia.
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MeSH Terms
Fructose / pharmacology
Galactose / pharmacology
Glucose / pharmacology
Glucosyltransferases / metabolism
Hexosyltransferases / metabolism*
Streptococcus / classification,  drug effects,  enzymology*
Sucrose / pharmacology*
Reg. No./Substance:
26566-61-0/Galactose; 30237-26-4/Fructose; 50-99-7/Glucose; 57-50-1/Sucrose; EC 2.4.1.-/Glucosyltransferases; EC 2.4.1.-/Hexosyltransferases; EC

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