Document Detail


Secretion of fructosyltransferase by Streptococcus salivarius involves the sucrose-dependent release of the cell-bound form.
MedLine Citation:
PMID:  2141067     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Three strains of Streptococcus salivarius including a recent clinical isolate were found to possess Ca2(+)-dependent fructosyltransferase (FTF) activity. The extracellular FTF activity of cells grown on sucrose increased as much as 9-fold compared with cells grown on either glucose, fructose or galactose. This increase in activity was due not to induction of FTF by sucrose, but to the release of the cell-bound form of the enzyme. Studies with washed cells of S. salivarius ATCC 25975 showed that the extent of release of the cell-bound FTF activity was dependent upon the sucrose concentration up to 4 mM, at which concentration maximum release (95%) of cell-bound FTF occurred. Several lines of evidence suggested that either substrate binding or de novo synthesis of fructan is required for the release of the cell-bound FTF activity.
Authors:
C P Milward; N A Jacques
Related Documents :
20346347 - Nucleocytoplasmic shuttling of hexokinase ii in a cancer cell.
15283587 - Raman microscopic analysis of single microbial cells.
2982627 - Prolyl hydroxylase production can be uncoupled from the regulation of procollagen synth...
17874157 - Functional characterization of the microbial community in geothermally heated marine se...
8687117 - Establishment of a daunorubicin-resistant cell line which shows multi-drug resistance b...
18620267 - The phorbol ester tpa dramatically inhibits planarian regeneration.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of general microbiology     Volume:  136     ISSN:  0022-1287     ISO Abbreviation:  J. Gen. Microbiol.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-07-13     Completed Date:  1990-07-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375371     Medline TA:  J Gen Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  165-9     Citation Subset:  IM    
Affiliation:
Institute of Dental Research, United Dental Hospital of Sydney, Surry Hills, New South Wales, Australia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Fructose / pharmacology
Galactose / pharmacology
Glucose / pharmacology
Glucosyltransferases / metabolism
Hexosyltransferases / metabolism*
Kinetics
Streptococcus / classification,  drug effects,  enzymology*
Sucrose / pharmacology*
Chemical
Reg. No./Substance:
26566-61-0/Galactose; 30237-26-4/Fructose; 50-99-7/Glucose; 57-50-1/Sucrose; EC 2.4.1.-/Glucosyltransferases; EC 2.4.1.-/Hexosyltransferases; EC 2.4.1.9/inulosucrase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Aging increases the energetic demands of episodic memory: a cardiovascular analysis.
Next Document:  Analysis of influenza A virus temperature-sensitive mutants with mutations in RNA segment 8.