Document Detail


Secretion-and-capture cell-surface display for selection of target-binding proteins.
MedLine Citation:
PMID:  21402751     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
This report describes a new cell-surface display system, the Secretion and Capture Technology (SECANT™) platform, which relies on in vivo biotinylation of the protein of interest followed by its capture on the avidinated surface of the parent cell. Cell sorting techniques are then used to isolate clones that display target-binding protein. A distinguishing feature of this method is its ability to display complex proteins, such as full-length immunoglobulin G (IgG) antibodies, on living cells. In this proof-of-concept study, Saccharomyces cerevisiae cells that displayed Herceptin IgG were isolated from a 10 000-fold excess of cells that displayed a lysozyme-binding antibody.
Authors:
J A Rakestraw; D Aird; P M Aha; B M Baynes; D Lipovsek
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-14
Journal Detail:
Title:  Protein engineering, design & selection : PEDS     Volume:  -     ISSN:  1741-0134     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101186484     Medline TA:  Protein Eng Des Sel     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Celexion LLC, One Kendall Square, Suite 14302, Cambridge, MA 02139, USA.
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