Document Detail

Secretion of RANTES (CCL5) and interleukin-10 from mesenteric adipose tissue and from creeping fat in Crohn's disease: regulation by steroid treatment.
MedLine Citation:
PMID:  16911685     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Creeping fat represents a characteristic feature of Crohn's disease (CD) and adipose tissue is currently being recognized as a complex compartment secreting highly active molecules. Pro- or anti-inflammatory adipose tissue-derived secretory products (adipocytokines) might play a role in the pathogenesis of CD. METHODS: Adipose tissue specimens were obtained from creeping fat contiguous to the involved intestine of 10 patients with CD. Mesenteric adipose tissue specimens resected in 13 patients with colon cancer (CC) and in seven patients with diverticulitis (DIV) served as controls. Three fat tissue specimens per well and n = 6-8 wells per patient were incubated ex vivo for 24 h. The release of regulated on activation, T-cell expressed and secreted (RANTES) and interleukin (IL)-10 into the supernatant was measured by ELISA. RESULTS: Both RANTES and IL-10 secretion could be demonstrated from total adipose tissue explants. The RANTES secretion is increased from creeping fat in CD (3691 +/- 597 pg/g fat per 24 h) when compared to mesenteric adipose tissue from patients with CC (1690 +/- 191 pg/g fat per 24 h; P < 0.0001) or DIV (1672 +/- 336 pg/g fat per 24 h; P < 0.0001). In contrast, IL-10 secretion is downregulated significantly only in patients with DIV (1418 +/- 180 pg/g fat per 24 h; P = 0.016) when compared to CC patients (2368 +/- 259 pg/g fat per 24 h). Crohn's disease patients receiving steroids had a higher secretion rate of RANTES and IL-10. CONCLUSIONS: Both RANTES and IL-10 secretion can be detected from mesenteric adipose tissue and from creeping fat. The elevated RANTES and IL-10 secretion from creeping fat in CD is not due to a CD-specific effect but caused by steroid treatment.
Andreas Schäffler; Alois Fürst; Christa Büchler; Gisela Paul; Gerhard Rogler; Jürgen Schölmerich; Hans Herfarth
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of gastroenterology and hepatology     Volume:  21     ISSN:  0815-9319     ISO Abbreviation:  J. Gastroenterol. Hepatol.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-16     Completed Date:  2007-05-24     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8607909     Medline TA:  J Gastroenterol Hepatol     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  1412-8     Citation Subset:  IM    
Department of Internal Medicine I, University of Regensburg, Regensburg, Germany.
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MeSH Terms
Adipose Tissue / secretion*
C-Reactive Protein / metabolism
Chemokine CCL5 / secretion*
Chemokines / secretion
Colonic Neoplasms / metabolism
Crohn Disease / drug therapy*,  metabolism*,  pathology,  physiopathology
Cytokines / secretion
Diverticulitis / metabolism
Interleukin-10 / secretion*
Mesentery / metabolism
Middle Aged
Steroids / therapeutic use*
Reg. No./Substance:
0/Chemokine CCL5; 0/Chemokines; 0/Cytokines; 0/Steroids; 130068-27-8/Interleukin-10; 9007-41-4/C-Reactive Protein

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