| Secretion of novel SEL1L endogenous variants is promoted by ER stress/UPR via endosomes and shed vesicles in human cancer cells. | |
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MedLine Citation:
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PMID: 21359144 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe here two novel endogenous variants of the human endoplasmic reticulum (ER) cargo receptor SEL1LA, designated p38 and p28. Biochemical and RNA interference studies in tumorigenic and non-tumorigenic cells indicate that p38 and p28 are N-terminal, ER-anchorless and more stable relative to the canonical transmembrane SEL1LA. P38 is expressed and constitutively secreted, with increase after ER stress, in the KMS11 myeloma line and in the breast cancer lines MCF7 and SKBr3, but not in the non-tumorigenic breast epithelial MCF10A line. P28 is detected only in the poorly differentiated SKBr3 cell line, where it is secreted after ER stress. Consistently with the presence of p38 and p28 in culture media, morphological studies of SKBr3 and KMS11 cells detect N-terminal SEL1L immunolabeling in secretory/degradative compartments and extracellularly-released membrane vesicles. Our findings suggest that the two new SEL1L variants are engaged in endosomal trafficking and secretion via vesicles, which could contribute to relieve ER stress in tumorigenic cells. P38 and p28 could therefore be relevant as diagnostic markers and/or therapeutic targets in cancer. |
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Authors:
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Monica Cattaneo; Lavinia Vittoria Lotti; Simone Martino; Massimo Alessio; Antonio Conti; Angela Bachi; Renato Mariani-Costantini; Ida Biunno |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-02-17 |
Journal Detail:
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Title: PloS one Volume: 6 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2011 |
Date Detail:
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Created Date: 2011-03-01 Completed Date: 2011-09-01 Revised Date: 2013-03-28 |
Medline Journal Info:
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Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States |
Other Details:
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Languages: eng Pagination: e17206 Citation Subset: IM |
Affiliation:
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Institute for Biomedical Technologies, National Research Council, Milan, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line, Tumor Cells, Cultured Endoplasmic Reticulum / metabolism* Endosomes / metabolism*, physiology HeLa Cells Humans Neoplasms / genetics, metabolism*, pathology Protein Isoforms / genetics, secretion Protein Transport / physiology Proteins / genetics, secretion* Secretory Vesicles / metabolism*, pathology Stress, Physiological / physiology Tumor Markers, Biological / genetics, secretion Unfolded Protein Response / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Protein Isoforms; 0/Proteins; 0/SEL1L protein, human; 0/Tumor Markers, Biological |
| Comments/Corrections | |
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