Document Detail

Secretion of 6beta-hydroxycortisol by normal human adrenals and adrenocortical adenomas.
MedLine Citation:
PMID:  12798499     Owner:  NLM     Status:  MEDLINE    
Although 6beta-hydroxycortisol (6betaOHF) is usually considered a cortisol metabolite produced by the liver, a few reports suggest that it may also originate from extrahepatic sources. To examine whether human adrenal cells are capable of 6beta-hydroxylating cortisol, we measured 6betaOHF secretion with a radioimmunoassay method in isolated human adrenal cell systems obtained from three normal adrenals, four nonhyperfunctioning adrenocortical adenomas, two adrenal adenomas causing Cushing's syndrome, and five aldosterone (Aldo)-producing adenomas. Cells were examined both under basal conditions and after stimulation with adrenocorticotrophic hormone (ACTH). In addition, 6betaOHF concentrations were determined in inferior vena cava and suprarenal vein plasma samples obtained from the side of nonhyperfunctioning adrenal adenomas (five patients) and aldosterone-producing adenomas (five patients). Under basal incubation conditions, 6betaOHF secretion, expressed as a percent of cortisol secretion, was between 0.5 and 2.0% in normal adrenal cells, between 1.0 and 7% in cells from nonhyperfunctioning adenomas, 12 and 15% in cells from Cushing's syndrome patients, and between 2.6 and 3.9% in cells from aldosterone-producing adenomas. In these cells, increasing doses of ACTH produced a dose-dependent stimulation of both 6betaOHF and cortisol secretion. The 6betaOHF concentration in suprarenal vein samples obtained from the side of adenomas was markedly increased; the suprarenal vein/inferior vena cava 6betaOHF ratios were 13.1+/-2.1 (mean+/-S.E.) in the case of nonhyperfunctioning adenomas and 17.8+/-4.5 in the case of aldosterone-producing adenomas. These results firmly suggest that 6betaOHF is not only a hepatic metabolite, but also a secretory product of human adrenals and that similarly to cortisol, its secretion may be controlled by ACTH.
Nikolette Szucs; Ibolya Varga; Attila Patócs; Miklós Tóth; Edit Gláz; Károly Rácz
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Steroids     Volume:  68     ISSN:  0039-128X     ISO Abbreviation:  Steroids     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-06-11     Completed Date:  2004-02-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404536     Medline TA:  Steroids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  477-82     Citation Subset:  IM    
Gastroenterological and Endocrinological Research Group, 2nd Department of Medicine, Faculty of Medicine, Semmelweis University, Szentkirályi 46, Budapest H-1088, Hungary.
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MeSH Terms
Adrenal Cortex / cytology,  drug effects,  secretion*
Adrenal Cortex Neoplasms / metabolism,  pathology,  secretion*
Adrenocorticotropic Hormone / pharmacology
Aldosterone / biosynthesis
Dose-Response Relationship, Drug
Hydrocortisone / analogs & derivatives*,  secretion*
Reg. No./Substance:
50-23-7/Hydrocortisone; 52-39-1/Aldosterone; 53-35-0/6 beta-hydroxycortisol; 9002-60-2/Adrenocorticotropic Hormone

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