Document Detail


Secreted protein lipocalin-2 promotes microglial M1 polarization.
MedLine Citation:
PMID:  23207546     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Activated macrophages are classified into two different forms: classically activated (M1) or alternatively activated (M2) macrophages. The presence of M1/M2 phenotypic polarization has also been suggested for microglia. Here, we report that the secreted protein lipocalin 2 (LCN2) amplifies M1 polarization of activated microglia. LCN2 protein (EC(50) 1 μg/ml), but not glutathione S-transferase used as a control, increased the M1-related gene expression in cultured mouse microglial cells after 8-24 h. LCN2 was secreted from M1-polarized, but not M2-polarized, microglia. LCN2 inhibited phosphorylation of STAT6 in IL-4-stimulated microglia, suggesting LCN2 suppression of the canonical M2 signaling. In the lipopolysaccharide (LPS)-induced mouse neuroinflammation model, the expression of LCN2 was notably increased in microglia. Primary microglial cultures derived from LCN2-deficient mice showed a suppressed M1 response and enhanced M2 response. Mice lacking LCN2 showed a markedly reduced M1-related gene expression in microglia after LPS injection, which was consistent with the results of histological analysis. Neuroinflammation-associated impairment in motor behavior and cognitive function was also attenuated in the LCN2-deficient mice, as determined by the rotarod performance test, fatigue test, open-field test, and object recognition task. These findings suggest that LCN2 is an M1-amplifier in brain microglial cells.-Jang, E., Lee, S., Kim, J.-H., Kim, J.-H., Seo, J.-W., Lee, W.-H., Mori, K., Nakao, K., Suk, K. Secreted protein lipocalin-2 promotes microglial M1 polarization.
Authors:
Eunha Jang; Shinrye Lee; Jong-Heon Kim; Jae-Hong Kim; Jung-Wan Seo; Won-Ha Lee; Kiyoshi Mori; Kazuwa Nakao; Kyoungho Suk
Related Documents :
23551896 - Contrast ultrasound for the quantification of deep vein thrombosis in living mice - eff...
23867766 - Reversal of novelty-induced hyperlocomotion and hippocampal c-fos expression in glua1 k...
22974546 - Effect of the miaoyao fanggan sachet-derived isorhamnetin on tlr2/4 and nkp46 expressio...
23537646 - Voltage-dependent n-type ca(2+) channels in endothelial cells contribute to oxidative s...
7131856 - Resistance of mice to secondary infection with schistosoma japonicum, with special refe...
18346986 - 5-lipoxygenase, but not 12/15-lipoxygenase, contributes to degeneration of retinal capi...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-4
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  -     ISSN:  1530-6860     ISO Abbreviation:  FASEB J.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
*Department of Pharmacology, Brain Science and Engineering Institute, CMRI, Kyungpook National University School of Medicine, and.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Synthesis and cytotoxicity of (+/-)-7,9-dideoxy-pancratistatin analogues.
Next Document:  XAF1 contributes to dengue virus-induced apoptosis in vascular endothelial cells.