| Secretagogue-induced proteolysis of lung spectrin in alveolar epithelial type II cells. | |
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MedLine Citation:
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PMID: 1420319 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Incubation of isolated rat alveolar epithelial type II cells with secretagogues (calcium ionophore, ATP or terbutaline) resulted in rapid proteolysis of lung spectrin and appearance of multiple proteolytic products which showed immunoreactivity with an antibody against human erythrocyte spectrin. These proteolytic products were similar to those generated from erythrocyte spectrin or cultured lung tumor cells (A549 cells) incubated with purified calpain. Furthermore, incubation of alveolar type II cells with a calpain-specific inhibitor modulated the secretagogue-induced proteolysis of lung spectrin. Thus, stimulation of secretion appeared to activate endogenous calpain in type II cells, suggesting that calpain-mediated proteolysis of a submembranous cytoskeletal protein could play an important role in the secretory process. |
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Authors:
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U J Zimmerman; D W Speicher; A B Fisher |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1137 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1992 Oct |
Date Detail:
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Created Date: 1992-12-08 Completed Date: 1992-12-08 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 127-34 Citation Subset: IM |
Affiliation:
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Institute for Environmental Medicine, University of Pennsylvania Medical School, Philadelphia 19104. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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physiology Animals Blotting, Western Calcimycin / pharmacology Calpain / antagonists & inhibitors, physiology* Cells, Cultured Enzyme Activation Epithelial Cells Epithelium / metabolism Humans Lung / metabolism* Membrane Fusion Pulmonary Alveoli / cytology, metabolism* Pulmonary Surfactants / metabolism Rats Spectrin / metabolism* Terbutaline / pharmacology Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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HL 19737/HL/NHLBI NIH HHS; HL 38794/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Pulmonary Surfactants; 12634-43-4/Spectrin; 23031-25-6/Terbutaline; 52665-69-7/Calcimycin; 56-65-5/Adenosine Triphosphate; EC 3.4.22.-/Calpain |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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