Document Detail


Secondary lymphoid tissue as an important site for WU polyomavirus infection in immunocompetent children.
MedLine Citation:
PMID:  21678449     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The polyomaviruses KI and WU (KIPyV and WUPyV) have been identified in respiratory specimens from children with acute respiratory infections, which suggests the respiratory tract as a possible site of infection. However, the persistence of infection in the lymphoid system is unknown. Fresh samples (n = 211) of tonsils, adenoids, and peripheral blood mononuclear cells (PBMCs) from 83 immunocompetent children (mean age 4.8 years) were tested for amplification of the KIPyV VP1 and WUPyV VP2 genes. The known BK and JC polyomaviruses and the lymphotropic human herpesvirus (HHV)-6 were also investigated by quantitative real-time PCR and direct sequencing. In addition, 98 nasopharyngeal swabs collected from children (mean age 6.2 years) affected by seasonal influenza-like illness were tested. Of the lymphoid tissues, 34.9% were positive for WUPyV, 4.8% for BK virus, and 33.8% for HHV-6. KIPyV and JC virus were not detected in these specimens. None of the polyomaviruses were detected in PBMCs. Among the nasopharyngeal samples, the prevalence of WUPyV was 27.5%, although 70% of the positive samples were co-infected with at least one of the following respiratory viruses: influenza virus, adenovirus, and respiratory syncytial virus. Phylogenetic analysis revealed high sequence homology (99%) between lymphoid- and nasopharynx-derived WUPyV strains. These results suggest that the tonsils and adenoids of immunocompetent children are a reservoir for WUPyV infection; probably due to the respiratory route of transmission. In addition, the prevalence of WUPyV was high among the children, and the virus was identified more frequently in older children than during the first years of life. J. Med. Virol. 83:1446-1450, 2011. © 2011 Wiley-Liss, Inc.
Authors:
Manola Comar; Nunzia Zanotta; Tatiana Rossi; Giorgio Pelos; Pierlanfranco D'Agaro
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of medical virology     Volume:  83     ISSN:  1096-9071     ISO Abbreviation:  J. Med. Virol.     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-06-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705876     Medline TA:  J Med Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1446-50     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
Affiliation:
Department of Reproductive, Developmental and Public Health Sciences, UCO Hygiene and Preventive Medicine, Institute of Child Health IRCCS Burlo Garofolo, University of Trieste, Trieste, Italy. comar@burlo.trieste.it.
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