Document Detail

Secondary intervention with aminoguanidine retards the progression of diabetic retinopathy in the rat model.
MedLine Citation:
PMID:  7672485     Owner:  NLM     Status:  MEDLINE    
Primary prevention with aminoguanidine-an inhibitor of advanced glycation end product (AGE) formation--has been successfully employed to prevent diabetic retinopathy in the rat. However, it is unknown whether inhibition of AGE formation is still effective in a secondary intervention strategy. The present study addresses this question by comparing secondary intervention with aminoguanidine with syngeneic islet transplantation in the rat model. After 6 months of diabetes, one group was treated with aminoguanidine (50 mg/100 ml drinking water; D-AG) while another group received syngeneic transplantation of collagenase-ficoll isolated islets by intraportal injection (Tx). After an additional 4 months, both groups were compared to a normal (NC 10) and diabetic (DC 10) control group. Retinal autofluorescence was increased 2.5-fold after 6 months and increased 3.7-fold after 10 months of diabetes (p < 0.001). Aminoguanidine and islet Tx retarded the further accumulation of autofluorescence equally (p < 0.001 vs DC 10), although the values were higher than those observed in DC at 6 months (p < 0.001). Diabetes was associated with a 2.7-fold increase in acellular capillaries after 6 months and a 4.1-fold increase after 10 months. Treatment with aminoguanidine or islet Tx reduced but did not completely attenuate the progression of vascular occlusion (p < 0.001 vs DC 10; D-AG vs DC 6, p < 0.05; Tx vs DC 6, p < 0.01). Both treatments reduced endothelial proliferation (22.4% after 10 months; p < 0.001) and completely arrested pericyte dropout (40% after 10 months; p < 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)
H P Hammes; D Strödter; A Weiss; R G Bretzel; K Federlin; M Brownlee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diabetologia     Volume:  38     ISSN:  0012-186X     ISO Abbreviation:  Diabetologia     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-10-13     Completed Date:  1995-10-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0006777     Medline TA:  Diabetologia     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  656-60     Citation Subset:  IM    
Third Medical Department, Justus-Liebig-University, Giessen, Germany.
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MeSH Terms
Administration, Oral
Arterioles / drug effects,  pathology
Blood Glucose / metabolism
Body Weight / drug effects
Diabetes Mellitus, Experimental / physiopathology*,  surgery
Diabetic Retinopathy / physiopathology,  prevention & control*
Glycosylation End Products, Advanced / antagonists & inhibitors
Guanidines / administration & dosage,  therapeutic use*
Islets of Langerhans Transplantation*
Rats, Inbred Lew
Retinal Vessels / drug effects,  pathology
Transplantation, Isogeneic
Reg. No./Substance:
0/Blood Glucose; 0/Glycosylation End Products, Advanced; 0/Guanidines; 79-17-4/pimagedine

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