| Second harmonic generation imaging as a potential tool for staging pregnancy and predicting preterm birth. | |
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MedLine Citation:
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PMID: 20459265 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We use second harmonic generation (SHG) microscopy to assess changes in collagen structure of murine cervix during cervical remodeling of normal pregnancy and in a preterm birth model. Visual inspection of SHG images revealed substantial changes in collagen morphology throughout normal gestation. SHG images collected in both the forward and backward directions were analyzed quantitatively for changes in overall mean intensity, forward to backward intensity ratio, collagen fiber size, and porosity. Changes in mean SHG intensity and intensity ratio take place in early pregnancy, suggesting that submicroscopic changes in collagen fibril size and arrangement occur before macroscopic changes become evident. Fiber size progressively increased from early to late pregnancy, while pores between collagen fibers became larger and farther apart. Analysis of collagen features in premature cervical remodeling show that changes in collagen structure are dissimilar from normal remodeling. The ability to quantify multiple morphological features of collagen that characterize normal cervical remodeling and distinguish abnormal remodeling in preterm birth models supports future studies aimed at development of SHG endoscopic devices for clinical assessment of collagen changes during pregnancy in women and for predicting risk of preterm labor which occurs in 12.5% of all pregnancies. |
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Authors:
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Meredith L Akins; Katherine Luby-Phelps; Mala Mahendroo |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomedical optics Volume: 15 ISSN: 1560-2281 ISO Abbreviation: J Biomed Opt Publication Date: 2010 Mar-Apr |
Date Detail:
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Created Date: 2010-05-12 Completed Date: 2010-09-13 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 9605853 Medline TA: J Biomed Opt Country: United States |
Other Details:
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Languages: eng Pagination: 026020 Citation Subset: IM |
Affiliation:
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University of Texas Southwestern Medical Center, Department of Obstetrics and Gynecology, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cervix Uteri / metabolism*, ultrastructure* Female Fibrillar Collagens / analysis*, ultrastructure* Mice Microscopy, Fluorescence / methods* Pregnancy Premature Birth / diagnosis*, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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R01HD043154/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fibrillar Collagens |
| Comments/Corrections | |
Erratum In:
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J Biomed Opt. 2010 May-Jun;15(3):039802 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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