Document Detail

Second harmonic generation imaging as a potential tool for staging pregnancy and predicting preterm birth.
MedLine Citation:
PMID:  20459265     Owner:  NLM     Status:  MEDLINE    
We use second harmonic generation (SHG) microscopy to assess changes in collagen structure of murine cervix during cervical remodeling of normal pregnancy and in a preterm birth model. Visual inspection of SHG images revealed substantial changes in collagen morphology throughout normal gestation. SHG images collected in both the forward and backward directions were analyzed quantitatively for changes in overall mean intensity, forward to backward intensity ratio, collagen fiber size, and porosity. Changes in mean SHG intensity and intensity ratio take place in early pregnancy, suggesting that submicroscopic changes in collagen fibril size and arrangement occur before macroscopic changes become evident. Fiber size progressively increased from early to late pregnancy, while pores between collagen fibers became larger and farther apart. Analysis of collagen features in premature cervical remodeling show that changes in collagen structure are dissimilar from normal remodeling. The ability to quantify multiple morphological features of collagen that characterize normal cervical remodeling and distinguish abnormal remodeling in preterm birth models supports future studies aimed at development of SHG endoscopic devices for clinical assessment of collagen changes during pregnancy in women and for predicting risk of preterm labor which occurs in 12.5% of all pregnancies.
Meredith L Akins; Katherine Luby-Phelps; Mala Mahendroo
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  15     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:    2010 Mar-Apr
Date Detail:
Created Date:  2010-05-12     Completed Date:  2010-09-13     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  026020     Citation Subset:  IM    
University of Texas Southwestern Medical Center, Department of Obstetrics and Gynecology, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA.
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MeSH Terms
Cervix Uteri / metabolism*,  ultrastructure*
Fibrillar Collagens / analysis*,  ultrastructure*
Microscopy, Fluorescence / methods*
Premature Birth / diagnosis*,  metabolism*
Grant Support
Reg. No./Substance:
0/Fibrillar Collagens
Erratum In:
J Biomed Opt. 2010 May-Jun;15(3):039802

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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