Document Detail


Second generation of cycloSal-pronucleotides with esterase-cleavable sites: the "lock-in"-concept.
MedLine Citation:
PMID:  15043139     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A conceptual extension of the cycloSal-pronucleotide approach is presented. The characteristic feature of the new cycloSal-derivatives of the anti-HIV active nucleoside analogue d4T 1 is the incorporation of an enzymatically cleavable carboxylic ester moiety with the intention to trap the triesters inside cells ("lock-in"-concept). CycloSal-triesters bearing different ester groups in the 3-or 5-position of the cycloSal-moiety are described. Surprisingly, only acetyl-and levulinyl esters are cleaved readily in CEM cell extracts while alkyl esters were found to be stable. Nevertheless, in in-vitro anti-HIV assays most of the compounds achieve the thymidine-kinase bypass, thus proving that they act at least as nucleotide delivery systems.
Authors:
Chris Meier; Manuel F H Ruppel; Dalibor Vukadinović; Jan Balzarini
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nucleosides, nucleotides & nucleic acids     Volume:  23     ISSN:  1525-7770     ISO Abbreviation:  Nucleosides Nucleotides Nucleic Acids     Publication Date:  2004  
Date Detail:
Created Date:  2004-03-26     Completed Date:  2004-10-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100892832     Medline TA:  Nucleosides Nucleotides Nucleic Acids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  89-115     Citation Subset:  IM    
Affiliation:
Institute of Organic Chemistry, University of Hamburg, Hamburg, Germany. chris.meier@chemie.uni-hamburg.de
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MeSH Terms
Descriptor/Qualifier:
Esterases / metabolism*
HIV Infections / drug therapy*
HIV-1 / drug effects*
Humans
Nucleotides / chemical synthesis,  metabolism,  pharmacology*
Chemical
Reg. No./Substance:
0/Nucleotides; EC 3.1.-/Esterases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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