Document Detail


Seasonal upregulation of fatty acid transporters in flight muscles of migratory white-throated sparrows (Zonotrichia albicollis).
MedLine Citation:
PMID:  19717675     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Endurance flights of birds, some known to last several days, can only be sustained by high rates of fatty acid uptake by flight muscles. Previous research in migratory shorebirds indicates that this is made possible in part by very high concentrations of cytosolic heart-type fatty acid binding protein (H-FABP), which is substantially upregulated during migratory seasons. We investigated if H-FABP and other components of muscle fatty acid transport also increase during these seasons in a passerine species, the white-throated sparrow (Zonotrichia albicollis). Fatty acid translocase (FAT/CD36) and plasma-membrane fatty acid binding protein (FABPpm) are well characterized mammalian proteins that facilitate transport of fatty acid through the muscle membrane, and in this study they were identified for the first time in birds. We used quantitative PCR to measure mRNA of FAT/CD36, FABPpm and H-FABP and immunoblotting to measure protein expression of FABPpm and H-FABP in the pectoralis muscles of sparrows captured in migratory (spring, fall) and non-migratory (winter) seasons. During migratory seasons, mRNA expression of these genes increased 70-1000% above wintering levels, while protein expression of H-FABP and FABPpm increased 43% and 110% above wintering levels. Activities of key metabolic enzymes, 3-hydroxyacyl-CoA-dehydrogenase (HOAD), carnitine palmitoyl transferase II (CPT II), and citrate synthase (CS) also increased (90-110%) in pectoralis muscles of migrant birds. These results support the hypothesis that enhanced protein-mediated transport of fatty acids from the circulation into muscle is a key component of the changes in muscle biochemistry required for migration in birds.
Authors:
Jay T McFarlan; Arend Bonen; Christopher G Guglielmo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of experimental biology     Volume:  212     ISSN:  1477-9145     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-31     Completed Date:  2009-12-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2934-40     Citation Subset:  IM    
Affiliation:
Department of Biology, University of Western Ontario, London, ON, Canada. mcfarlan@uoguelph.ca
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MeSH Terms
Descriptor/Qualifier:
Animal Migration / physiology*
Animals
Fatty Acid-Binding Proteins / genetics,  metabolism*
Fatty Acids / metabolism
Female
Flight, Animal / physiology*
Humans
Male
Molecular Sequence Data
Muscle, Skeletal / metabolism*
Physical Endurance / physiology
Protein Isoforms / metabolism*
RNA, Messenger / metabolism
Seasons*
Sparrows
Up-Regulation*
Chemical
Reg. No./Substance:
0/Fatty Acid-Binding Proteins; 0/Fatty Acids; 0/Protein Isoforms; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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