Document Detail


Seasonal dynamics of harmful algae in outer Oslofjorden monitored by microarray, qPCR, and microscopy.
MedLine Citation:
PMID:  23325054     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Monitoring of marine microalgae is important to predict and manage harmful algal blooms. Microarray Detection of Toxic ALgae (MIDTAL) is an FP7-funded EU project aiming to establish a multi-species microarray as a tool to aid monitoring agencies. We tested the suitability of different prototype versions of the MIDTAL microarray for the monthly monitoring of a sampling station in outer Oslofjorden during a 1-year period. Microarray data from two different versions of the MIDTAL chip were compared to results from cell counts (several species) and quantitative real-time PCR (qPCR; only Pseudochattonella spp.). While results from generation 2.5 microarrays exhibited a high number of false positive signals, generation 3.3 microarray data generally correlated with microscopy and qPCR data, with three important limitations: (1) Pseudo-nitzschia cells were not reliably detected, possibly because cells were not sufficiently retained during filtration or lysed during the extraction, and because of low sensitivity of the probes; (2) in the case of samples with high concentrations of non-target species, the sensitivity of the arrays was decreased; (3) one occurrence of Alexandrium pseudogonyaulax was not detected due to a 1-bp mismatch with the genus probe represented on the microarray. In spite of these shortcomings our data demonstrate the overall progress made and the potential of the MIDTAL array. The case of Pseudochattonella - where two morphologically similar species impossible to separate by light microscopy were distinguished - in particular, underlines the added value of molecular methods such as microarrays in routine phytoplankton monitoring.
Authors:
Simon M Dittami; Vladyslava Hostyeva; Elianne Sirnæs Egge; Jessica U Kegel; Wenche Eikrem; Bente Edvardsen
Related Documents :
23686434 - The role of dimerisation and nuclear transport in the hes1 gene regulatory network.
11009024 - Liquid adsorption chromatography of polyethers: experiments and simulation.
19589674 - Biosorption of nonylphenol on dead biomass of rhizopus arrhizus encapsulated in chitosa...
20888664 - Effectiveness of nanoscale zero-valent iron for treatment of a pce-dnapl source zone.
11385844 - Advanced monitoring and control of anaerobic wastewater treatment plants: software sens...
23628254 - Prediction of coagulating and noncoagulating milk samples using mid-infrared spectroscopy.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-17
Journal Detail:
Title:  Environmental science and pollution research international     Volume:  -     ISSN:  1614-7499     ISO Abbreviation:  Environ Sci Pollut Res Int     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9441769     Medline TA:  Environ Sci Pollut Res Int     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Marine Biology, Department of Biology, University of Oslo, P.O. Box 1066, Blindern, 0316, Oslo, Norway, simon.dittami@gmail.com.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Nickel-catalyzed regio- and diastereoselective intermolecular three-component coupling of oxabicycli...
Next Document:  A multi-approach monitoring of particulate matter, metals and PAHs in an urban street canyon.