Document Detail


Seasonal restructuring of the ground squirrel gut microbiota over the annual hibernation cycle.
MedLine Citation:
PMID:  23152108     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Many hibernating mammals suspend food intake during winter, relying solely on stored lipids to fuel metabolism. Winter fasting in these species eliminates a major source of degradable substrates to support growth of gut microbes, which may affect microbial community structure and host-microbial interactions. We explored the effect of the annual hibernation cycle on gut microbiotas using deep sequencing of 16S rRNA genes from ground squirrel cecal contents. Squirrel microbiotas were dominated by members of the phyla Bacteroidetes, Firmicutes, and Verrucomicrobia. UniFrac analysis showed that microbiotas clustered strongly by season, and maternal influences, diet history, host age, and host body temperature had minimal effects. Phylogenetic diversity and numbers of operational taxonomic units were lowest in late winter and highest in the spring after a 2-wk period of refeeding. Hibernation increased relative abundance of Bacteroidetes and Verrucomicrobia, phyla that contain species capable of surviving on host-derived substrates such as mucins, and reduced relative abundance of Firmicutes, many of which prefer dietary polysaccharides. Hibernation reduced cecal short-chain fatty acid and ammonia concentrations, and increased and decreased concentrations of acetate and butyrate, respectively. These results indicate that the ground squirrel microbiota is restructured each year in a manner that reflects differences in microbial preferences for dietary vs. host-derived substrates, and thus the competitive abilities of different taxa to survive in the altered environment in the hibernator gut.
Authors:
Hannah V Carey; William A Walters; Rob Knight
Related Documents :
24613098 - Termites in the hominin diet: a meta-analysis of termite genera, species and castes as ...
15285508 - Predicting effective caloric value of nonnutritive factors: i. pellet quality and ii. p...
23448018 - Biological control of eotetranychus lewisi and tetranychus urticae (acari: tetranychida...
10685018 - Review of the toxicologic properties of medium-chain triglycerides.
2965088 - The anatomy of adipose tissue in captive macaca monkeys and its implications for human ...
12048128 - Change in saturated fat intake is associated with progression of carotid and femoral in...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-11-14
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  304     ISSN:  1522-1490     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-02     Completed Date:  2013-03-07     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R33-42     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acetates / analysis
Ammonia / analysis
Animals
Bacteria / genetics,  isolation & purification
Bacteroidetes / genetics,  isolation & purification
Biodiversity
Butyrates / analysis
Fatty Acids, Volatile / analysis
Female
Gastrointestinal Tract / microbiology*
Genes, rRNA
Hibernation*
Metagenome / genetics*
Phylogeny
Sciuridae / microbiology*,  physiology*
Seasons*
Verrucomicrobia / genetics,  isolation & purification
Grant Support
ID/Acronym/Agency:
T32 GM008759/GM/NIGMS NIH HHS; //Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/Acetates; 0/Butyrates; 0/Fatty Acids, Volatile; 7664-41-7/Ammonia
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Antiproliferative effects of kisspeptin?10 depend on artificial GPR54 (KISS1R) expression levels.
Next Document:  Role of the transient receptor potential vanilloid type 1 channel in renal inflammation induced by l...