Document Detail

Searching for virulence Burkholderia pseudomallei genes by immunoscreening the lambda ZAPII expressed genomic library.
MedLine Citation:
PMID:  15115093     Owner:  NLM     Status:  MEDLINE    
The lambdaZAP II expressed genomic library of B. pseudomallei was screened with pooled melioidosis serum preabsorbed with E. coli host cell. The positive clones were detected by using protein A-CDP-star chemiluminescence. All of 14 positive clones reacted with only the pooled absorbed melioidosis serum and not the pooled absorbed normal serum when tested with the plaque dot blot analysis. The expressed genes were detected by using a combination of immunoscreening, bioinformatics and molecular biology. At least six in vivo expressed genes were identified by this approach. Two were well known virulent genes, gmhA (a capsule biosynthetic gene) and bipD (type III secretion protein gene). Another two were genes coded for conserved hypothetical protein. The last two isolated genes were groEL (a chaperonine protein gene), and a gene encoding transmembrane protein.
Siroj Jitsurong; Chintana Chirathaworn; Nat F Brown; Ifor R Beacham; Somying Tumwasorn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Southeast Asian journal of tropical medicine and public health     Volume:  34     ISSN:  0125-1562     ISO Abbreviation:  Southeast Asian J. Trop. Med. Public Health     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2004-04-29     Completed Date:  2004-05-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0266303     Medline TA:  Southeast Asian J Trop Med Public Health     Country:  Thailand    
Other Details:
Languages:  eng     Pagination:  810-21     Citation Subset:  IM    
Inter-Department of Medical Microbiology, Graduate School and Prince of Songkla University, Songkhla, Thailand.
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MeSH Terms
Bacterial Proteins / genetics*
Bacteriophage lambda
Burkholderia pseudomallei / genetics*,  pathogenicity*
Chemiluminescent Measurements
Cloning, Molecular
Gene Expression Profiling
Genomic Library
Melioidosis / microbiology*
Virulence / genetics
Reg. No./Substance:
0/Bacterial Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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