| Searching biomarker candidates in serum using multidimensional native chromatography. II Method evaluation with Alport syndrome and severe inflammation. | |
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MedLine Citation:
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PMID: 18952508 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Biomarker search using multidimensional native liquid fractionation of serum in microplates was evaluated. From different donors, homologous sample fractions with UV absorbance depending on state of illness were selected, and their constituents were identified and quantitated by MS. Analysis of sera of patients with Alport syndrome and severe inflammation proved the reliability of the method by confirming characteristic alterations. Moreover, 23 new marker candidates were detected for Alport syndrome, some of them being involved in matrix degradation and repair, and 33 new candidates for severe inflammation, among them alpha1B-glycoprotein cysteine-rich secretory protein and an apparently low molecular-weight albumin variant. |
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Authors:
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Adrienne Baum; Michael Pohl; Stefan Kreusch; Gerhard A Cumme; Günter Ditze; Joachim Misselwitz; Michael Kiehntopf; Lene Udby; Andreas Meier-Hellmann; Heidrun Rhode |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2008-10-14 |
Journal Detail:
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Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: 876 ISSN: 1570-0232 ISO Abbreviation: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-25 Completed Date: 2009-02-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 31-40 Citation Subset: IM |
Affiliation:
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Institute of Biochemistry, Friedrich Schiller University, Medical Faculty, Jena, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Biological Markers / blood* Chemical Fractionation / methods Child Female Glycoproteins / blood Humans Immunoglobulins / blood Male Nephritis, Hereditary / blood* Sepsis / blood* Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods |
| Chemical | |
Reg. No./Substance:
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0/A1BG protein, human; 0/Biological Markers; 0/Glycoproteins; 0/Immunoglobulins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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