Document Detail

Search for appropriate experimental methods to create stable hind-limb ischemia in mouse.
MedLine Citation:
PMID:  21302240     Owner:  NLM     Status:  In-Data-Review    
Objective: Stable animal models for refractory peripheral arterial disease are not established. A standardized animal model of hind-limb ischemia is required upon searching effective treatment for this condition. The aim of the study is to verify previously used hind-limb ischemia models to find a standard method. Methods: Using Balb/ca mice six various methods of inducing hind-limb ischemia were applied and two weeks after operation degree of ischemic damage were examined. Six methods include V group, A group, AV group, A-strip group, AV-strip group and Prox-A group (refer the text). Results: Degree of ischemia was evaluated macroscopically by judging toes, foot, knee, and total hind-limb necrosis. We found that severity of damage was markedly different among different methods. Furthermore the severity of necrosis was not uniform even in the same method group. Conclusions: The A-strip group in which the femoral artery from the bifurcation of the deep femoral artery to the saphenous artery was stripped appears to be suitable as a stable severe ischemia model. The A group in which the femoral artery were cut just below the bifurcation of the deep femoral artery appears to be suitable as a chronic mild ischemia model.
Takako Goto; Naoto Fukuyama; Akira Aki; Kazuo Kanabuchi; Koji Kimura; Hiroyuki Taira; Etsuro Tanaka; Noriaki Wakana; Hidezo Mori; Hiroshi Inoue
Publication Detail:
Type:  Journal Article     Date:  2006-09-20
Journal Detail:
Title:  The Tokai journal of experimental and clinical medicine     Volume:  31     ISSN:  0385-0005     ISO Abbreviation:  Tokai J. Exp. Clin. Med.     Publication Date:  2006  
Date Detail:
Created Date:  2011-02-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7704186     Medline TA:  Tokai J Exp Clin Med     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  128-32     Citation Subset:  IM    
Department of Surgery, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan
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