| Screening of toxicity biomarkers for methionine excess in rats. | |
| | |
MedLine Citation:
|
PMID: 16702345 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Although many animal studies have reported that dietary excess of methionine causes toxic changes including growth suppression and hemolytic anemia, the biochemical mechanism and biomarkers for methionine toxicity have not been well elucidated. The present study aimed to identify toxicity biomarkers from plasma metabolites in rats fed excessive methionine. Young growing rats were fed graded doses of additional methionine for 2 wk. Cluster analysis of multivariate correlations was performed on the physiological and toxicity variables with plasma metabolites detected by GC/MS, amino acid analyzer, and thiol-specific analysis. Indicative variables for hemolysis such as splenic nonheme iron content and plasma bilirubin were grouped in the same cluster as many methionine metabolites. Homocysteine and some undefined metabolites in this cluster were found to be strong discriminators between nontoxic and toxic levels of methionine intake. Product-to-precursor ratios of each methionine metabolite demonstrated that excessive methionine intake caused a marked decrease only in the ratio of cystathionine to homocysteine, suggesting that metabolism from homocysteine to cystathionine would be rate limiting in the disposal of excessive methionine. Collectively from these results, homocysteine appeared to be the most plausible biomarker to assess methionine excess as a surrogate marker both for toxicity and for setting a metabolic upper limit. |
| | |
Authors:
|
Sakino Toue; Riho Kodama; Michiko Amao; Yasuko Kawamata; Takeshi Kimura; Ryosei Sakai |
Related Documents
:
|
6307195 - The effect of bis-pyridinium oximes on neuromuscular blockade induced by highly toxic o... 3820865 - Chitin is an effective material for sutures. 9061855 - Selected lesions of dioxin in laboratory rodents. 7172405 - The effect of timing of a single injection on the toxicity of methotrexate in the rat. 1281375 - Lack of significant unspecific effects of hoe 140 and other novel bradykinin antagonist... 18042385 - Spermicidal bacteriocins: lacticin 3147 and subtilosin a. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The Journal of nutrition Volume: 136 ISSN: 0022-3166 ISO Abbreviation: J. Nutr. Publication Date: 2006 Jun |
Date Detail:
|
Created Date: 2006-05-16 Completed Date: 2006-07-10 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0404243 Medline TA: J Nutr Country: United States |
Other Details:
|
Languages: eng Pagination: 1716S-1721S Citation Subset: IM |
Affiliation:
|
Ajinomoto Co., Inc., Institute of Life Sciences, Kawasaki, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids
/
blood Analysis of Variance Animals Bilirubin / blood Biological Markers / blood* Cystathionine / blood Diet Gas Chromatography-Mass Spectrometry Glycine / administration & dosage Hemolysis Homocysteine / blood Iron / blood, metabolism Male Methionine / administration & dosage, toxicity* Rats Rats, Inbred F344 Serine / administration & dosage Spleen / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Biological Markers; 454-28-4/Homocysteine; 56-40-6/Glycine; 56-45-1/Serine; 56-88-2/Cystathionine; 63-68-3/Methionine; 635-65-4/Bilirubin; 7439-89-6/Iron |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Assessing the effects of high methionine intake on DNA methylation.
Next Document: Toxicity of methionine in humans.