Document Detail

Screening of ten plant species for metaphase chromosome preparation in adult mosquitoes (Diptera: Culicidae) using an inoculation technique.
MedLine Citation:
PMID:  10593098     Owner:  NLM     Status:  MEDLINE    
The screening of 10 plant species (Aloe barbadensis Mill., Asparagus officinalis L., As. plumosus Bak., As. racemosus Willd., As. sprengeri Regel, Codyline fruticosa Goppert, Dracaena loureiri Gagnep., Gloriosa superba L., Hemerocallis flava L., and Sansevieria cylindrica Bojer) for colchicine-like substance(s) using a mosquito cytogenetic assay revealed that a 1% solution of dried Gl. superba rhizome extracted in 0.85% sodium chloride solution could be used instead of a 1% colchicine in Hanks' balanced salt solution. The metaphase rates and average number of metaphase chromosomes per positive mosquito of Aedes aegypti (L.) after intrathoracic inoculation with 1% Gl. superba-extracted solution were 100% and 29.80 in females, and 90% and 25.78 in males, whereas the inoculation with 1% colchicine solution yielded 100 and 90% metaphase rates, and 20.90 and 12.22 average number of metaphase chromosomes per positive mosquito in females and males, respectively. The application of Gl. superba-extracted solution for metaphase chromosome preparation in other mosquito genera and species [e.g., Culex quinquefasciatus Say, Toxorhynchites splendens (Wiedemann), and Anopheles vagus (Döenitz)] also has yielded the satisfactory results.
A Jitpakdi; W Choochote; D Insun; P Tippawangkosol; P Keha; B Pitasawat
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medical entomology     Volume:  36     ISSN:  0022-2585     ISO Abbreviation:  J. Med. Entomol.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  2000-01-07     Completed Date:  2000-01-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375400     Medline TA:  J Med Entomol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  892-5     Citation Subset:  IM    
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Thailand.
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MeSH Terms
Chromosomes / drug effects*
Colchicine / isolation & purification,  pharmacology*
Culicidae / genetics*
Mutagenicity Tests
Mutagens / pharmacology*
Plant Extracts / chemistry,  pharmacology*
Plants, Medicinal
Species Specificity
Reg. No./Substance:
0/Mutagens; 0/Plant Extracts; 64-86-8/Colchicine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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