Document Detail


Screening of rat mesenchymal stem cell behaviour on polydimethylsiloxane stiffness gradients.
MedLine Citation:
PMID:  21989230     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Substrate stiffness is emerging as an effective tool for the regulation of cell behaviours such as locomotion, proliferation and differentiation. In order to explore the potential application of this biophysical tool, material platforms displaying lateral and continuously graded stiffness are advantageous since they allow the systematic exploration of adherent cell response to substrate stiffness and the tuning of the material to elicit the desired cell behaviour. Here, we demonstrate a simple approach towards the fabrication of polydimethylsiloxane (PDMS) stiffness gradients (with an indentation modulus of 190kPa-3.1MPa across a 12mm distance) by means of a temperature gradient during curing. We then apply these stiffness gradients to the screening of osteogenic differentiation in rat mesenchymal stem cells (rMSCs). Our proof-of-principle results show that mineralization of rMSCs is strongly dependent on the PDMS substrate stiffness, but is also influenced by the display of extracellular matrix proteins preadsorbed on the gradients. This screening capability holds tremendous potential for the design of improved implant materials and tissue engineering scaffolds.
Authors:
Peng-Yuan Wang; Wei-Bor Tsai; Nicolas H Voelcker
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-28
Journal Detail:
Title:  Acta biomaterialia     Volume:  -     ISSN:  1878-7568     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-10-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101233144     Medline TA:  Acta Biomater     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Ltd.
Affiliation:
Centre for NanoScale Science and Technology, School of Chemical and Physical Sciences, Flinders University, Adelaide, South Australia, Australia; Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
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