Document Detail


Screening procedures for clenbuterol residue determination in raw swine livers using lateral-flow assay and enzyme-linked immunosorbent assay.
MedLine Citation:
PMID:  18468049     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Clenbuterol, which may cause symptoms of increased heart rate, muscular tremors, headache, nausea, and muscular cramps in patients, has been prohibited for consumption in many countries including the European Union, the United States, and China. A rapid lateral-flow strip assay was developed in our laboratory, and results obtained with this assay were compared with those obtained with a commercial enzyme-linked immunosorbent assay (ELISA) kit for the screening of clenbuterol in raw swine liver. A total of 128 swine livers were acquired from five local markets and prepared for analysis by the lateral-flow strip assay and ELISA. Analysis was completed in 10 min with the lateral-flow strip assay and in 90 min with the ELISA. In parallel with the ELISA, the rapid detection strip produced no false-negative results but had a false-positive rate of 6.3%. Cross-reactivity of the strip was assessed and was negative after tests with clenbuterol analogues such as terbutaline, salbutamol, ractopamine, ritodrine, and fenoterol. These data suggest that a lateral-flow strip assay can be used safely as a screening method as part of a clenbuterol residue surveillance program and should be a valuable tool in the food safety field, especially in developing countries.
Authors:
Wei H Lai; Daniel Y C Fung; Yang Xu; Yong H Xiong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food protection     Volume:  71     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-05-12     Completed Date:  2008-06-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  865-9     Citation Subset:  IM    
Affiliation:
Key Laboratory of Food Science, Nanchang University, Ministry of Education, Nanchang 330047, China. talktolaiwh@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Adrenergic beta-Agonists / adverse effects,  analysis
Animals
Antibodies, Monoclonal
Clenbuterol / adverse effects,  analysis*
Consumer Product Safety
Drug Residues / analysis*
Enzyme-Linked Immunosorbent Assay / methods*
False Negative Reactions
False Positive Reactions
Food Contamination / analysis*
Humans
Liver / chemistry*
Reagent Kits, Diagnostic*
Reagent Strips
Reproducibility of Results
Sensitivity and Specificity
Swine
Chemical
Reg. No./Substance:
0/Adrenergic beta-Agonists; 0/Antibodies, Monoclonal; 0/Reagent Kits, Diagnostic; 0/Reagent Strips; 37148-27-9/Clenbuterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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