| Screening phosphatidylcholine biomarkers in mouse liver extracts from a hypercholesterolemia study using ESI-MS and chemometrics. | |
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MedLine Citation:
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PMID: 19030846 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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When fed a high-fat, high-cholesterol diet (HFD), homozygous LDL receptor knockout mice exhibit extremely high levels of plasma cholesterol that are expected to influence liver metabolism. One step in the investigation of potential hepatic alterations was the analysis of organic extracts of livers from these and control mice by electrospray mass spectrometry (ESI-MS). Chemometrics (bioinformatics) analysis shows that the sample spectra cluster into two groups: one from mice with plasma cholesterol levels in excess of 900 mg dL(-1) and one from animals with cholesterol levels of 60-250 mg dL(-1). The loadings plot of the first PC in the principal-components analysis (PCA) reveals the chemical basis for clustering, i.e., biomarkers present at different concentrations in the different groups. The exact masses of the key peaks in this loadings plot indicate these species are phosphatidylcholines (PtdChos). This assignment is confirmed by tandem MS. Partial least-squares (PLS) with variable selection shows that the spectra are well correlated with plasma total cholesterol, HDL cholesterol, and triglyceride (TG) levels. |
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Authors:
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Li Yang; Richard Bennett; John Strum; Benjamin B Ellsworth; David Hamilton; Matthew Tomlinson; Rudolf W Wolf; Michael Housley; Brad A Roberts; Jesse Welsh; Bruce J Jackson; Steven G Wood; Carole L Banka; Craig D Thulin; Matthew R Linford |
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Publication Detail:
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Type: Journal Article Date: 2008-11-23 |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: 393 ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2008-12-19 Completed Date: 2009-02-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 643-54 Citation Subset: IM |
Affiliation:
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Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers / analysis* Cluster Analysis Computational Biology* Hypercholesterolemia / blood* Liver / chemistry* Mice Mice, Inbred C57BL Phosphatidylcholines / analysis* Receptors, LDL / deficiency Reproducibility of Results Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization* |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Phosphatidylcholines; 0/Receptors, LDL |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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