Document Detail

Screening of natural medicines that efficiently activate neurite outgrowth in PC12 cells in C2C12-cultured medium.
MedLine Citation:
PMID:  22361883     Owner:  NLM     Status:  MEDLINE    
We have studied the effects of natural medicines on neurite outgrowth in PC12D cells in a cultured medium of C2C12 cells. Derived from mouse myoblasts, the C2C12 cells secrete neurotrophic factors including nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3). The secretion of these neurotrophins from C2C12 cells stimulate neurite outgrowth in PC12D cells. We have screened a total of 120 samples and found five natural medicines: Trichosanthes Root, Asiasarum Root, Lycium Bark, Sinomenium Stem, and Dictamni radicis Cortex, that enhance the activity of C2C12-cultured medium to stimulate neurite outgrowth in PC12D cells. These natural medicines promoted not only neurite outgrowth but also stabilized the neurite formation in PC12D cells for several days. RT-PCR analysis showed that NGF was significantly increased with Trichosanthes and Lycium Bark. However, BDNF was slightly decreased with Lycium Bark, Sinomenium Stem, and Dictamni radicis Cortex. NT-3 was increased slightly by all of these natural medicines except Sinomenium Stem. All these five natural medicines significantly increased the number and length of neurites in PC12D cells in co-culture with C2C12 cells.
Tadayoshi Uezato; Eiji Sato; Naoyuki Miura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomedical research (Tokyo, Japan)     Volume:  33     ISSN:  1880-313X     ISO Abbreviation:  Biomed. Res.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-24     Completed Date:  2012-06-18     Revised Date:  2012-09-11    
Medline Journal Info:
Nlm Unique ID:  8100317     Medline TA:  Biomed Res     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  25-33     Citation Subset:  IM    
Department of Biochemistry, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Hamamatsu 431-3192, Japan.
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MeSH Terms
Brain-Derived Neurotrophic Factor / metabolism,  secretion
Cells, Cultured
Coculture Techniques
Culture Media, Conditioned / chemistry*
Drugs, Chinese Herbal / pharmacology*
Nerve Growth Factor / metabolism
Nerve Growth Factors / metabolism,  secretion
Neurites / drug effects*,  metabolism*
Neurotrophin 3 / metabolism,  secretion
PC12 Cells
Plants, Medicinal / chemistry
Reg. No./Substance:
0/Brain-Derived Neurotrophic Factor; 0/Culture Media, Conditioned; 0/Drugs, Chinese Herbal; 0/Nerve Growth Factors; 0/Neurotrophin 3; 9061-61-4/Nerve Growth Factor

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