| Screening microorganisms for insulin binding reveals binding by Burkholderia multivorans and Burkholderia cenocepacia and novel attachment of insulin to Aeromonas salmonicida via the A-layer. | |
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MedLine Citation:
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PMID: 22171975 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Exposure to microorganisms is considered an environmental factor which can contribute to Type 1 diabetes. Insulin binding proteins on microorganisms may induce production of antibodies which can react with the human insulin receptor (HIR) with possible consequences in developing a diabetic autoimmune response against HIR and insulin. The interaction of insulin with microorganisms was studied by screening forty-five microbial species for their ability to bind insulin. Binding assays were performed using labelled insulin to identify insulin binding components on the microbes. Burkholderia multivorans and Burkholderia cenocepacia isolated from cystic fibrosis (CF) patients and the fish pathogen A.salmonicida were the only strains of those tested which showed insulin-binding components on their cell surfaces. Further work with A.salmonicida suggested that the insulin binding activity of A.salmonicida is due to the A-layer. A mutant of A.salmonicida lacking the A-layer showed binding, but at a much reduced rate suggesting another insulin binding component in addition to the high affinity of the A-protein. Soluble protein lysates were subjected to western ligand blotting using peroxidase-labelled insulin to detect insulin binding proteins (IBPs). Two positive IBPs were apparent at approximately 30 & 20 kDa in lysates from Burkholderia strains, but no IBP was detected in A.salmonicida lysates. |
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Authors:
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Raid B Nisr; A John Moody; Martyn L Gilpin |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-15 |
Journal Detail:
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Title: FEMS microbiology letters Volume: - ISSN: 1574-6968 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. |
Affiliation:
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Centre for Research in Translational Biomedicine, School of Biomedical and Biological Sciences, University of Plymouth, Drake Circus, Plymouth, PL4 8AA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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