| Screening method for detection of immediate amino acid decarboxylases - producing bacteria implicated in food poisoning. | |
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MedLine Citation:
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PMID: 22041756 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Assessment of amino acid decarboxylase activity can be conducted using tubed broth or plated agar. In this study, the test was carried out in microtitre plates containing lysine, ornithine, arginine, tyrosine, tryptophan, phenylalanine or histidine as biogenic amine precursors. Møller decarboxylase base broth (MDB) with or without 1% of a known amino acid were added to wells of a 96 well-microtitre plate. The wells were inoculated with Escherichia coli, Klebsiella pneumoniae, Acinetobacter anitratus or Staphylococcus aureus to the final concentration of 6.0 x 10(7)cfu/ml and incubated at 35ºC. The absorbance of the culture broth was read at 570 nm at 0, 1.0, 2.0, 3.0, 4.0, 5.5, 6.5 and 7.5 hour. Comparison of means of A'(570) between 0 hour and a specified incubation time was determined statistically. Positive decarboxylase activities were detected in the media inoculated with E. coli and K. pneumoniae in less than 6 hours. The current method is suitable for immediate producers of amino acid decarboxylase enzymes. It costs less as it uses less amino acid and it has the potential to be used for screening aliquots of food materials for amino acid decarboxylase activities. |
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Authors:
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H Husniza; A R Mohd Fuat; B Vimala; H M Ghazali |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Tropical biomedicine Volume: 28 ISSN: 0127-5720 ISO Abbreviation: Trop Biomed Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-11-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8507086 Medline TA: Trop Biomed Country: Malaysia |
Other Details:
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Languages: eng Pagination: 351-61 Citation Subset: IM |
Affiliation:
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Nutrition Unit, Institute for Medical Research, Kuala Lumpur, Malaysia. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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