Document Detail


Screening for the hepatitis C virus in some dental clinics in Alexandria, Egypt.
MedLine Citation:
PMID:  23196884     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
BACKGROUND/OBJECTIVES: Individuals can be exposed to the hepatitis C virus (HCV) infection through inadequately or improperly sterilized medical or dental equipment. The aim of this study was to detect HCV RNA in the dental setting in Alexandria, Egypt.
MATERIALS AND METHODS: The study included 100 samples collected from five dental clinics (A-E) in Alexandria. The samples were collected from critical, semicritical, and noncritical instruments during different periods of the day (morning, mid-day, end of the day). Samples were subjected to a reverse transcriptase-PCR for the detection of HCV RNA.
RESULTS: HCV RNA was detected in 18% (18 out of 100) of the instrument samples tested. Two positive HCV RNA samples were collected from semicritical instruments in clinic B, whereas 16 positive HCV RNA samples were collected from clinic D (eight samples from critical, six samples from semicritical, and two samples from noncritical instruments). There was a statistically significant difference between clinics B and D in terms of the samples collected in the morning and those collected at the end of the day.
CONCLUSION AND RECOMMENDATIONS: HCV RNA as detected by PCR was found in a considerable percent of instruments' samples (18%). Most of the positive HCV RNA samples (16 out of 18 samples) obtained from instruments were among those collected from clinic D. This clinic used only glutaraldehyde as a method of sterilization. Therefore, proper infection control measures, including sterilization and disinfection should be strictly adopted.
Authors:
Mona H Hashish; Heba S Selim; Soraya A Elshazly; Hanan H Diab; Naguiba M Elsayed
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of the Egyptian Public Health Association     Volume:  87     ISSN:  0013-2446     ISO Abbreviation:  J Egypt Public Health Assoc     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7505602     Medline TA:  J Egypt Public Health Assoc     Country:  England    
Other Details:
Languages:  eng     Pagination:  109-15     Citation Subset:  IM    
Affiliation:
aDepartment of Microbiology, High Institute of Public Health bDepartment of Oral Medicine, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
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