Document Detail


Screening of gluten avenins in foods by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
MedLine Citation:
PMID:  9821333     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The first procedure capable of analysing gluten avenins in gluten-free food samples aimed at the diet control of coeliac patients is described. The method is based on the direct observation of the characteristic avenin mass pattern, around 20-30 kDa, as revealed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS). The mass range where avenin signals appear is free from mass peaks arising from wheat gliadin, barley hordein and rye secalin protein components, which are also toxic to coeliac patients. Therefore, avenins can easily be screened in complex formula food samples elaborated with mixtures of wheat, barley, rye and oats. In addition, a procedure to quantify avenins in food samples is described on the basis of avenin mass area measurement with a detection limit of 0.4 mg of avenins per 100 g of food.
Authors:
E Camafeita; E Méndez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of mass spectrometry : JMS     Volume:  33     ISSN:  1076-5174     ISO Abbreviation:  J Mass Spectrom     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1998-12-22     Completed Date:  1998-12-22     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9504818     Medline TA:  J Mass Spectrom     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1023-8     Citation Subset:  IM    
Affiliation:
Unidad de Análisis Estructural de Proteinas, Centro Nacional de Biotecnología, CSIC, Madrid, Spain.
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MeSH Terms
Descriptor/Qualifier:
Avena sativa / chemistry
Ethanol
Food Analysis / methods*
Glutens / chemistry*
Indicators and Reagents
Plant Proteins / chemistry*
Prolamins
Solvents
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 0/Plant Proteins; 0/Prolamins; 0/Solvents; 64-17-5/Ethanol; 8002-80-0/Glutens

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