Document Detail


Screening of dairy yeast strains for probiotic applications.
MedLine Citation:
PMID:  15545365     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To evaluate the potential of yeasts of dairy origin as probiotics, we tested 8 species including Candida humilis, Debaryomyces hansenii, Debaryomyces occidentalis, Kluyveromyces lactis, Kluyveromyces lodderae, Kluyveromyces marxianus, Saccharomyces cerevisiae, and Yarrowia lipolytica, isolated from commercial blue cheese and kefir. Strains were randomly selected from each species and tested for their ability to adhere to human enterocyte-like Caco-2 cells in culture. Among the 8 species, K. lactis showed higher adhesive ability than K. marxianus, K. lodderae, and D. hansenii. The other 4 species were poorly adhesive. All species other than K. marxianus and C. humilis were resistant to acidic conditions. In the presence of bile acid, growth inhibition was undetectable when incubation was carried out at 27 degrees C; however, it was evident for C. humilis and a strain of D. occidentalis when incubated at 37 degrees C. Moreover, the influence of proteinase treatment of living cells of K. lactis and K. lodderae on their adhesion to Caco-2 cells was evaluated. Although a slight reduction was recognized when K. lactis was treated with proteinase K, the influence of intestinal protease treatments of pepsin followed by trypsin was negligible. These results indicated that a proteinaceous factor was unlikely to be involved in adhesion of K. lactis and K. lodderae to Caco-2 cells. No stimulation of IL-8 synthesis by Caco-2 cells was recognized in the presence of K. lactis. In conclusion, K. lactis was the most attractive to continue study for use as probiotic microorganisms.
Authors:
H Kumura; Y Tanoue; M Tsukahara; T Tanaka; K Shimazaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of dairy science     Volume:  87     ISSN:  0022-0302     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-11-16     Completed Date:  2005-04-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4050-6     Citation Subset:  IM    
Affiliation:
Laboratory of Dairy Science, Research Group of Animal Product Science, Division of Bioresources and Product Science, Graduate School of Agriculture, Hokkaido University, Sapporo-shi 060-8589, Japan. kumura@anim.agr.hokudai.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Caco-2 Cells / microbiology
Cell Adhesion / physiology*
Cheese / microbiology
Dairy Products / microbiology*
Food Microbiology
Health Food / microbiology*
Humans
Hydrogen-Ion Concentration
Kluyveromyces / isolation & purification,  physiology
Probiotics / isolation & purification*
Saccharomyces / isolation & purification,  physiology
Saccharomycetales / isolation & purification,  physiology
Temperature
Yarrowia / isolation & purification,  physiology
Yeasts / isolation & purification*,  physiology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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