Document Detail

Screening and characterization of Isochrysis strains and optimization of culture conditions for docosahexaenoic acid production.
MedLine Citation:
PMID:  23423326     Owner:  NLM     Status:  Publisher    
Isochrysis is a genus of marine unicellular microalgae that produces docosahexaenoic acid (DHA, C22:6), a very long chain polyunsaturated fatty acid (PUFA) of significant health and nutritional value. Mass cultivation of Isochrysis for DHA production for human consumption has not been established due to disappointing low DHA productivity obtained from commonly used Isochrysis strains. In this study, 19 natural Isochrysis strains were screened for DHA yields and the results showed that the cellular DHA content ranged from 6.8 to 17.0 % of total fatty acids with the highest DHA content occurring in the exponential growth phase. Isochrysis galbana #153180 exhibited the greatest DHA production potential and was selected for further investigation. The effects of different light intensities, forms, and concentrations of nitrogen, phosphorus, and salinity on growth and DHA production of I. galbana #153180 were studied in a bubble column photobioreactor (PBR). Under favorable culture conditions, I. galbana #153180 contained DHA up to 17.5 % of total fatty acids or 1.7 % of cell dry weight. I. galbana #153180 was further tested in outdoor flat-plate PBRs varying in light path length, starting cell density (SCD), and culture mode (batch versus semicontinuous). When optimized, record high biomass and DHA productivity of I. galbana #153180 of 0.72 g L(-1) day(-1) and 13.6 mg L(-1) day(-1), or 26.4 g m(-2) day(-1) and 547.7 mg m(-2) day(-1), respectively, were obtained, suggesting that I. galbana #153180 may be a desirable strain for commercial production of DHA.
Jin Liu; Milton Sommerfeld; Qiang Hu
Related Documents :
24430136 - Glutamic acid production bybacillus isolates from nigerian fermented vegetable proteins.
23673436 - Nano-micro-architectural acidic silica prepared from iron oxide of bacterial origin.
24135586 - Influence of monochromatic light on quality traits, nutritional, fatty acid, and amino ...
38376 - Physiological studies on phymatotrichum omnivorum. ix. synthesis of indole acetic acid ...
16161606 - Adsorption of acetic acid on ice: experiments and molecular dynamics simulations.
21191576 - Clinical significance of uric acid dihydrate in urinary stones.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-20
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  -     ISSN:  1432-0614     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Laboratory for Algae Research and Biotechnology, Department of Applied Sciences and Mathematics, Arizona State University Polytechnic Campus, Mesa, AZ, 85212, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Enhancement of volatile thiol release of Saccharomyces cerevisiae strains using molecular breeding.
Next Document:  Comparison of pyruvate decarboxylases from Saccharomyces cerevisiae and Komagataella pastoris (Pichi...